背景：Pyometra是一种常见的与大肠杆菌相关的慢性疾病。最初的子宫积脓治疗包括经验性抗菌治疗，其适用性应通过抗菌药敏试验确认。抗菌素耐药性是兽医学的主要健康问题，使监测研究必不可少。我们的目标是确定从健康犬和子宫积脓犬获得的大肠杆菌分离株的敏感性曲线，并比较不同抗菌药物敏感性指南的应用。
方法：通过圆盘扩散测定74株大肠杆菌的抗菌药物敏感性曲线（ASP），使用兽医学中常用的六种抗菌药物。配置文件由CLSIVET01S评估，CLSIM100和EUCAST指南。β-内酰胺酶编码基因blaTEM,通过多重PCR检测blaSHV和blaOXA。通过在Luria-Bertani培养基中的薄膜形成测定来评估生物膜的生产能力。
结果：观察到阿莫西林/克拉维酸耐药频率的变化，头孢氨苄和头孢噻肟（29.7-54.1%，10.8-16.2%和1.4-4.1%，分别)。结果在临床和共生分离株之间略有不同，以及它们的生物膜形成能力。基因blaTEM,blaSHV和blaOXA的检出率为25.5%，11.8%和9.8%的分离株，分别。
结论：结果表明在兽医分离株中测定ASP的重要性，以及使用标准化和经过验证的测试方法和协调的解释标准的必要性。
BACKGROUND: Pyometra is a diestrual chronic disease frequently associated with Escherichia coli. Initial
pyometra treatment involves empiric antimicrobial therapy whose suitability should be confirmed by antimicrobial susceptibility testing. Antimicrobial resistance is a major health issue for veterinary medicine, rendering surveillance studies essential. Our goal was to determine the susceptibility profile of E. coli isolates obtained from healthy and
pyometra-presenting dogs and to compare the application of different antimicrobial susceptibility
guidelines.
METHODS: The antimicrobial susceptibility profile (ASP) of 74 E. coli isolates was determined by disk diffusion, using six antimicrobials commonly used in veterinary medicine. Profiles were assessed by CLSI VET01S, CLSI M100 and EUCAST
guidelines. β-lactamases-encoding genes blaTEM, blaSHV and blaOXA were detected by multiplex PCR. Biofilm production ability was evaluated by pellicle formation assays in Luria-Bertani medium.
RESULTS: Variations in the resistance frequency were observed for amoxicillin/clavulanic acid, cephalexin and cefotaxime (29.7-54.1%, 10.8-16.2% and 1.4-4.1%, respectively). Results varied slightly between clinical and commensal isolates, as well as their biofilm-forming ability. Genes blaTEM, blaSHV and blaOXA were detected in 25.5%, 11.8% and 9.8% of isolates, respectively.
CONCLUSIONS: Results show the importance of ASP determination in veterinary isolates and the need for using standardized and validated testing methods and harmonized interpretive criteria.
