目的:本研究的目的是评估绿脓素色素作为一种新型的酪氨酸酶活性化合物的功效,以及其对红癣毛癣菌的脱色效率,红癣菌可能是体癣的主要病原体。
方法:收集并鉴定真菌性体癣感染的50个拭子。测试了五种MDRPA分离株的绿脓苷生产水平。通过紫外光谱和FT-IR分析对纯化的提取的绿脓苷进行了表征。通过多巴色素微孔板测定了对酪氨酸酶的花青素活性。此外,通过径向生长技术检测了绿脓苷对红毛虫的抗皮肤癣菌活性。进行了体内新试验以评估作为替代的天然治疗化合物的绿脓苷对引起体癣的红毛虫的有效性和安全性。
结果:纯化的绿脓苷对酪氨酸酶和红色毛虫显示出非常显著的抑制活性。与氟康唑相比,在体内局部使用绿脓苷软膏治疗的效果表明,绿脓苷(MIC2000μg/ml)治愈体癣。在处理两周后,其在较高浓度(MIC3500μg/ml)下显示部分固化。此外,结果表明,通过测试绿脓苷软膏的安全性及其在皮肤治疗中的组织病理学功效,可以完全治愈并消失色素沉着,而没有任何明显的毒性作用。
结论:花青素色素可能是一种有前途的抗酪氨酸酶和新的活性化合物,这可能是体癣的主要病原体。事实上,如果将在实际药物中使用绿脓素次生代谢产物,它将支持对抗真菌感染的新型抗真菌天然药物的持续需求。
OBJECTIVE: The aim of this
study was to evaluate the efficiency of
pyocyanin pigment as a novel compound active against tyrosinase with its depigmentation efficiency for combating Trichophyton rubrum which could be a major causative agent of tinea corporis.
METHODS: Fifty swabs of fungal tinea corporis infections were collected and identified. Five MDRPA isolates were tested for their levels of
pyocyanin production. The purified extracted pyocyanin was characterized by UV spectrum and FT-IR analysis.
Pyocyanin activity against tyrosinase was determined by dopachrome micro-plate. In addition, the antidermatophytic activity of pyocyanin against T. rubrum was detected by radial growth technique. In vivo novel
trial was conducted to evaluate the efficiency and safety of
pyocyanin as an alternative natural therapeutic compound against T. rubrum causing tinea corporis.
RESULTS: Purified pyocyanin showed highly significant inhibitory activity against tyrosinase and T. rubrum. In vivo topical treatments with
pyocyanin ointment revealed the efficiency of
pyocyanin (MIC 2000 μg/ml) to cure tinea corporis compared to fluconazole, which showed a partial curing at a higher concentration (MIC 3500 μg/ml) after two weeks of treatment. In addition, the results revealed complete healing and disappear of hyperpigmentation by testing the safety of pyocyanin ointment and its histopathological efficiency in the skin treatment without any significant toxic effect.
CONCLUSIONS: Pyocyanin pigment could be a promising anti-tyrosinase and a new active compound against T. rubrum, which could be a major causative agent of tinea corporis. In fact, if pyocyanin secondary metabolite is going to be used in practical medication, it will support the continuous demand of novel antimycotic natural agents against troublesome fungal infections.