半营养植物病原体肉桂疫霉是鳄梨最具破坏性的病原体(PerseaamericanaMill。)和,因此,在行业中造成重大的年度损失。尽管鳄梨和肉桂毒力决定子对肉桂的抗性的分子基础一直是最近研究的主题。尚未尝试比较病原体和宿主在相互作用期间的转录组反应。在目前的研究中,通过双重RNA测序探索鳄梨和肉桂的转录组。通过包括在早期(接种后6、12和24小时)采样的易感砧木(R0.12)和部分抗性砧木(Dusa®)来寻求部分抗性的基础,hpi)和较晚的时间点(120hpi)。注意到在Dusa®和R0.12中发现的差异表达基因的数量存在显著差异,特别是在12和24hpi。这里,部分抗性砧木永久防御反应在6hpi开始,而易感砧木突然逆转。相反,基因本体论富集证实R0.12激活了与生长发育相关的通路,基本上使其在12和24hpi时的反应与模拟接种的对照没有什么不同。不出所料,几类肉桂P.cinnamomi效应基因在Dusa®和R0.12中差异表达。然而,它们的表达在砧木之间不同,表明肉桂可能会改变其基于砧木的效应器库的表达。根据观察到的一些差异,一些肉桂P.cinnamomi效应物被强调为进一步研究的潜在候选者。同样,研究了牛油果中的受体样激酶(RLK)和质外生蛋白酶编码基因,关注它们在不同砧木反应中的潜在作用。这项研究表明,Dusa®的部分抗性的基础是基于其在肉桂接种后的早期阶段做出适当反应的能力,第一道可诱导防御的重要组成部分,质蛋白酶和RLKs,可能对观察到的结果很重要。
The hemibiotrophic plant pathogen Phytophthora cinnamomi Rands is the most devastating pathogen of avocado (Persea americana Mill.) and, as such, causes significant annual losses in the industry. Although the molecular basis of P. cinnamomi resistance in avocado and P. cinnamomi virulence determinants have been the subject of recent research, none have yet attempted to compare the transcriptomic responses of both pathogen and host during their interaction. In the current study, the transcriptomes of both avocado and P. cinnamomi were explored by dual RNA sequencing. The basis for partial resistance was sought by the inclusion of both susceptible (R0.12) and partially resistant (Dusa®) rootstocks sampled at early (6, 12 and 24 hours post-inoculation, hpi) and late time-points (120 hpi). Substantial differences were noted in the number of differentially expressed genes found in Dusa® and R0.12, specifically at 12 and 24 hpi. Here, the partially resistant rootstock perpetuated defense responses initiated at 6 hpi, while the susceptible rootstock abruptly reversed course. Instead, gene ontology enrichment confirmed that R0.12 activated pathways related to growth and development, essentially rendering its response at 12 and 24 hpi no different from that of the mock-inoculated controls. As expected, several classes of P. cinnamomi effector genes were differentially expressed in both Dusa® and R0.12. However, their expression differed between rootstocks, indicating that P. cinnamomi might alter the expression of its effector arsenal based on the rootstock. Based on some of the observed differences, several P. cinnamomi effectors were highlighted as potential candidates for further research. Similarly, the receptor-like kinase (RLK) and apoplastic
protease coding genes in avocado were investigated, focusing on their potential role in differing rootstock responses. This study suggests that the basis of partial resistance in Dusa® is predicated on its ability to respond appropriately during the early stages following P. cinnamomi inoculation, and that important components of the first line of inducible defense, apoplastic proteases and RLKs, are likely to be important to the observed outcome.