昆虫毒理学分析不是常规毒理学分析的一部分。这项工作旨在介绍两个案例,以说明昆虫学样本作为互补基质在晚期腐败病例中识别物质的潜力。(病例1)一名妇女在14个月后被挖出,以确定确切的死亡原因。她在住院六周后因肠缺血和多器官衰竭而死亡。(案例2)一个女人的尸体,已知患有精神疾病,是在她的公寓里被发现的.身体的分解状态与几周(约6周)的验尸期一致。对病例#1的生物学和昆虫学样本进行了毒理学调查(头发,脂肪,大脑,和蛹)和2号病例(头发,骨头,苍蝇,和p)使用液相色谱与高分辨率质谱和串联质谱检测方法。在病例#1中,检测到几种药物和代谢物。特别是,蛹分析允许吗啡给药的客观化,而吗啡只在脂肪组中发现,但不在头发和大脑中。在2号病例中,蛹分析允许检测喹硫平的三种代谢物,果蝇分析允许检测到丙普罗胺,只在头发中检测到。总之,这两种情况下的p和果蝇分析补充了其他替代生物样品中获得的结果,这可以指导关于可能的死亡原因的假设。然而,额外的数据和病例报告将有助于评估昆虫毒理学在常规法医调查中的价值.
Entomotoxicological analysis is not part of routine toxicological analysis. This work aims to present two cases to illustrate the potential of entomological samples as complementary matrices to identify substances in cases of advanced putrefaction. (
Case#1) A woman wasexhumed after 14 months to ascertain the exact cause of death. She died after six weeks of hospitalization because of intestinal ischemia followed by multiorgan failure. (
Case#2) The corpse of a woman, known to have a psychiatric disorder, was discovered in her apartment. The state of decomposition of the body was consistent with a post-mortem period of several weeks (approximately 6 weeks). Toxicological investigations were performed in the biological and entomological samples of
case#1 (hair, adipocere, brain, and pupae) and of case#2 (hair, bone, flies, and pupae) using liquid chromatography with high-resolution mass spectrometry and tandem mass spectrometry detection methods. In
case#1, several drugs and metabolites were detected. In particular, the pupae analyses allowed the objectification of morphine administration, whereas morphine was only found in adipocere, but not in hair nor in brain. In case#2, the pupae analyses allowed the detection of three metabolites of quetiapine, and the flies analyses allowed the detection of valpromide, which was only detected in hair. In conclusion, the pupae and flies analyses in these two cases complemented the results obtained in the other alternative biological samples, which may guide hypotheses about the possible causes of death. Nevertheless, additional data and
case reports would be of benefit to assess the value of entomotoxicology in routine forensic investigations.