红眼病是具有不同病因的山羊的高度传染性疾病。老挝人民民主共和国的调查已将红眼病的典型眼部病变确定为常见病,然而,这还没有得到诊断证实,负责的病原体尚未确定。在沙湾拿吉省的70只山羊中进行了匹配的病例对照研究,老挝,检测导致红眼病的病原体,并对确定的病原体进行系统发育分析。从25个持有的山羊中收集了50个眼睛感染的山羊(病例)和50个未受影响的队列(对照)的配对样品。使用定量PCR测定在属和种水平上靶向已知的红眼病病原体来测试样品。病例山羊和对照山羊的病原体患病率如下:结膜支原体(分别为94%和74%,P=0.006,OR=5.5),衣原体(4%,10%),卵黄莫拉氏菌(30%,30%),牛莫拉氏菌(0%,0%)和博沃氏菌(0%,0%)。两组山羊中都存在结膜分枝杆菌,这表明老挝山羊是结膜分枝杆菌的携带者。然而,病例山羊的平均log10基因组拷贝数/μLDNA提取物显著高于对照山羊(P<0.05).因此,结膜分枝杆菌可能是老挝山羊红眼病的主要病原体,其携带者状态在角膜损伤或其他致病因素后转化为临床感染。在来自不同山羊和地区的样品中检测到的结膜分枝杆菌具有较低的遗传多样性。确定老挝山羊红眼病的原因将有助于设计适当的治疗和控制策略。
Pinkeye is a highly contagious disease of goats with different aetiologies. Surveys in Lao PDR have identified eye lesions typical of
pinkeye as a common condition, however, this has not been confirmed diagnostically, and the responsible pathogens have not been identified. A matched case-control
study was implemented in 70 goat holdings from Savannakhet province, Lao PDR, to detect agents causing
pinkeye and conduct phylogenetic analysis of the identified pathogens. Fifty eye swabs from goats with infected eyes (cases) and 50 paired samples from unaffected cohorts (controls) were collected from 25 holdings. Samples were tested using quantitative PCR assays targeting known
pinkeye pathogens at the genus and species levels. The prevalence of pathogens in case and control goats was as follows: Mycoplasma conjunctivae (94% and 74% respectively, P = 0.006, OR = 5.5), Chlamydia pecorum (4%, 10%), Moraxella ovis (30%, 30%), Moraxella bovis (0%, 0%) and Moraxella bovoculi (0%, 0%). M. conjunctivae was present in a high proportion of goats in both groups revealing that Lao goats are carriers of M. conjunctivae. However, the mean log10 genome copy number/µL of DNA extract was significantly higher in case goats than control goats (P < 0.05). Thus, M. conjunctivae is likely the principal causative agent of pinkeye in Lao goats with carrier status converting to clinical infection following corneal damage or other causative factors. M. conjunctivae detected in samples from different goats and districts showed low genetic diversity. Identifying the causes of pinkeye in Lao goats will assist in designing appropriate treatment and control strategies.