目的:在术中冷冻组织切片实验室(FS实验室)中,在组织载玻片上安装盖玻片的常规做法涉及使用基于二甲苯的安装剂,例如Pertex。然而,有毒蒸气对生物医学实验室科学家(BLS)和病理学家构成风险,他们在手术过程中为外科医生提供快速和紧急的诊断结果。我们的研究旨在评估无毒安装剂替代Pertex,优选具有适合病理学新数字时代的需求的快速固化时间。
方法:购买了五种无毒的安装剂,并通过六种不同的方案进行了测试,并与基于二甲苯的Pertex作为我们的黄金标准进行了比较。用光学显微镜,通过BLS评估组织切片的质量。安装剂,评估与Pertex相当,也由病理学家评估,因此,数字扫描并重新评估。
结果:EukittUV的协议,与黄金标准Pertex相比,EukittUVR-1和EukittUVR-2具有明显更多的伪影(气泡)(分别为p<0.0001,p=0.004和p<0.0001),并评估为替代不足。Neo-Mount和TissueMount在质量方面进行了评估,但是固化时间很长。TekSelectUV在质量和快速固化时间(方案<2分钟)方面均显示出有希望的结果。
结论:有毒的安装剂需要由卫生防护专业人员更换,数字病理学也正在彻底改变实验室。数字化FS实验室需要快速干燥/固化载玻片进行数字扫描。因此,FS实验室的替代品应具有无毒处理和快速固化时间的品质,和基于UV的安装剂可以解决这两个要求。
OBJECTIVE: In intraoperative frozen tissue section laboratories (FS laboratories) conventional practice for mounting coverslips on tissue slides involves the use of xylene-based mounting agents, such as Pertex. However, toxic vapours pose a risk to biomedical laboratory scientists (BLS) and pathologists who handle the wet slides to provide fast and urgent diagnostic results to surgeons during operations. Our study aims to evaluate non-toxic mounting agents to substitute Pertex, preferably with a fast curing time suitable for the demands of the new digital era in pathology.
METHODS: Five non-toxic mounting agents were purchased and tested through six different protocols and compared to xylene-based Pertex as our gold standard. With light microscopy, tissue slides were quality assessed by BLS. Mounting agents, which were evaluated comparable to Pertex, were also evaluated by a pathologist, hence scanned digitally and re-evaluated.
RESULTS: The protocols for Eukitt UV, Eukitt UV R-1 and Eukitt UV R-2 had significantly more artefacts (bubbles) compared to gold standard Pertex (p<0.0001, p=0.004 and p<0.0001, respectively) and assessed inadequate as replacements. Neo-Mount and Tissue Mount were assessed applicable regarding quality, but curing times were long. Tek Select UV showed promising results in both quality and fast curing time (protocol was <2 min).
CONCLUSIONS: Toxic mounting agents need replacement to health guard professionals, and also digital pathology is revolutionising laboratories. A digitalized FS laboratory requires fast dry/cured slides for digital scanning. Therefore, a substitute for the FS laboratory should have the qualities of being non-toxic to handle and having a fast curing time, and a UV-based mounting agent may solve both requirements.