目的:尿路感染(UTIs)是影响泌尿系统的常见感染,主要由细菌病原体引起,大肠杆菌是最常见的病原体。肾脏感染(例如,肾盂肾炎)严重且具有挑战性的治疗,由于特定的组织微环境。在这项研究中,分析了模拟肾脏环境的不同参数对肾盂肾炎处方抗生素对尿路病原菌生长的影响。
方法:为了研究模拟肾脏环境的不同因素的影响,我们测试了不同肾脏代表浓度的氯化钠和尿素的效果,和不同的pH值对厄他培南的疗效,左氧氟沙星,和头孢曲松.通过测定针对各种大肠杆菌菌株的最小抑制浓度(MIC)来评估有效性。
■研究表明,pH值显著影响左氧氟沙星的MIC值。酸化pH导致MIC值增加,而碱性pH具有相反的效果。氯化钠和尿素浓度的影响是菌株和抗生素特异性的。由于在这项研究中测试了三种不同的抗生素,需要进一步研究额外的抗生素.
结论:这些结果表明,肾脏内的理化状况可以极大地影响抗生素治疗肾盂肾炎的成功。因此,临床医生在选择和给药抗生素时考虑这些因素至关重要.需要进一步的研究来评估更广泛的抗生素和其他环境参数,更全面地了解肾脏环境如何影响抗菌活性。这些知识对于优化肾盂肾炎的治疗策略至关重要,最终改善患者预后。
结果:肾脏内的理化状况影响抗生素治疗肾盂肾炎的成功。我们的发现对于优化治疗策略至关重要,并将最终改善患者的预后。
OBJECTIVE: Urinary tract infections (UTIs) are common infections affecting the urinary system, predominantly caused by bacterial pathogens, with Escherichia coli being the most frequent pathogen. Infections of the kidney (eg,
pyelonephritis) are severe and challenging to treat, due to the specific tissue microenvironment. In this study, the influence of different parameters mimicking the kidney environment on the effectiveness of antibiotics prescribed for
pyelonephritis on the growth of uropathogenic strains was analyzed.
METHODS: To investigate the influence of different factors mimicking the kidney environment, we tested the effect of different kidney-representative concentrations of sodium chloride and urea, and different pH values on the efficacy of ertapenem, levofloxacin, and ceftriaxone. The effectiveness was assessed by determining the minimal inhibitory concentrations (MICs) against various E. coli strains.
UNASSIGNED: The study revealed that pH significantly influences the MIC values of levofloxacin. Acidification of the pH led to an increase of the MIC values, while an alkaline pH had the opposite effect. The influence of sodium chloride and urea concentrations was strain and antibiotic specific. Since three different antibiotics were tested in this study, further research with additional antibiotics is warranted.
CONCLUSIONS: These results suggest that the physicochemical conditions within the kidney can substantially influence the success of antibiotic therapy for
pyelonephritis. Therefore, it is crucial for clinicians to consider these factors when selecting and dosing antibiotics. Further research is needed to evaluate a broader range of antibiotics and additional environmental parameters, to develop a more comprehensive understanding of how the kidney environment affects antimicrobial activity. This knowledge will be vital in optimizing treatment strategies for
pyelonephritis, ultimately improving patient outcomes.
RESULTS: The physicochemical conditions within the kidney influence the success of antibiotic therapy for
pyelonephritis. Our findings are vital in optimizing treatment strategies and will ultimately improve patient outcomes.