诺卡氏菌属物种不像其他化脓性细菌那样快速复制,而腔心感染可能是高度致命的,特别是在免疫功能低下的患者中。这里,我们首次报道了使用OxfordNanoporeTechnologies\'MinION设备通过下一代测序(NGS)诊断的致命性诺卡氏菌菌血症肺炎和胸腔脓胸。通过基质辅助激光解吸电离-飞行时间质谱未鉴定出细菌。由于其设备成本低,短暂的周转时间,和便携式尺寸,牛津纳米孔技术MinION设备是常规临床微生物实验室中NGS的有用平台。
Nocardia species do not replicate as rapidly as other pyogenic bacteria and nocardial infections can be highly fatal, particularly in immunocompromised patients. Here, we present the first report of fatal Nocardia kroppenstedtii bacteremic pneumonia and empyema thoracis diagnosed by next-generation sequencing (NGS) using the Oxford Nanopore Technologies\' MinION device. The bacterium was not identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Due to its low equipment cost, short turn-around-time, and portable size, the Oxford Nanopore Technologies\' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.