由于外部性器官异常-阴蒂肥大,对8个月大的女性斯塔福德郡斗牛犬进行了临床检查。正如业主所说,这只母狗还没有发情。血清睾酮谱(3.39ng/ml),以及抗穆勒激素(24.0ng/ml),提示睾丸组织的存在.相反,17β-雌二醇的估计水平(24.6pg/ml)与正常的无发情值(5-10pg/ml)相比大约高出两倍。进行中线剖腹手术以检测生殖系统的颅骨部分。可见类似睾丸或卵睾丸的性腺(左)和发育不良的睾丸(右)。性腺的颅部附着在指示双侧附睾的结构上。切除下一个管状结构-输卵管以及发育不良子宫的粘附部分。组织学评估证实,所检查的性腺样品是具有改良的间质睾丸组织的睾丸。间质肥大主要由Leydig细胞形成。通过可疑附睾头部的横截面检查证实了它们的特征结构。此外,介绍了输卵管的特征结构。子宫由三个壁组成,其中子宫内膜增生,存在子宫内膜腺体。使用CFAY探针通过染色体分析未检测到Y染色体,并且SRY基因编码区(813bp)的扩增表明基因型78,XX;SRY阴性。SOX9基因外显子1-3的测序未显示外显子1和3的任何差异。相反,在SOX9外显子2序列中确定了一些变化:在位置103处G代替A;在位置115处C代替参考T;在位置138-140处GCG代替参考CGC;在位置161、164和167处T代替参考C。
An 8-month-old female Staffordshire bull terrier was clinically examined because of external sexual organs abnormality-clitoral hypertrophy. As stated by the owner, the female dog had not been in heat yet. Serum profile of testosterone (3.39 ng/ml), as well as an anti-Műllerian hormone (24.0 ng/ml), suggested the presence of testicular tissue. On the contrary, the estimated level of 17β-oestradiol (24.6 pg/ml) was approximately two times higher when compared with the normal anoestrus values (5-10 pg/ml). A midline laparotomy was performed to detect the cranial parts of the genital system. Gonads resembling testicle or ovotestis (left) and hypoplastic testicle (right) was visible. Cranial portion of gonads was attached to structures indicative of bilateral epididymidis. The next tubular structures-oviducts were resected along with adherent parts of a hypoplastic uterus. Histological evaluation confirmed that the examined gonad samples were testicles with modified interstitial testicular tissue. Hypertrophy of interstitial space was predominantly formed by Leydig cells. Examination of a cross-section through the head of suspected epididymidis confirmed their characteristic structures. In addition, the characteristic configuration of the oviducts was presented. The uterus consisted of three walls, in which the endometrium was hypoplastic with the presence of endometrial glands. No Y chromosome was detected by chromosomal analysis using CFA Y probe and the amplification of SRY-gene coding region (813 bp) indicated genotype 78, XX; SRY-negative. Sequencing of SOX9 gene exons 1-3 did not reveal any differences in exon 1 and 3. On the contrary, a few changes were determined in the SOX9 exon 2 sequences: G instead of A at position 103; C instead of reference T at position 115; GCG instead of reference CGC at position 138-140; T instead of reference C at positions 161, 164 and 167.