背景:口腔癌(OC)是临床实践中常见的恶性肿瘤。唾液测试是一种方便且无创的OC早期诊断技术。几种唾液细胞因子已被确定为OC的潜在生物标志物,包括IL-8,IL-6,TNF-α,IL-1β,IL-10尽管如此,OC诊断的最佳细胞因子仍无定论且存在很大争议.
方法:PubMed,Embase,WebofScience,全面检索CochraneLibrary数据库,以收集有关OC的所有病例对照研究。进行了荟萃分析,以比较唾液IL-8,IL-6,IL-10,TNF-α,和IL-1β在OC患者和健康对照中的表达。进行网络荟萃分析(NMA)以探讨这些唾液细胞因子在诊断OC中的准确性。
结果:这项分析包括40项研究,包括1280名OC和1254名健康对照者。显著较高水平的唾液IL-8、IL-6、TNF-α、IL-1β,与健康对照组相比,OC患者中观察到IL-10。NMA结果显示TNF-α对OC的诊断准确率最高,灵敏度为79%,特异性为92%,其次是IL-6(敏感性:75%,特异性:86%)和IL-8(灵敏度:80%,特异性:80%)。
结论:本研究提示IL-8、IL-6、IL-10、TNF-α、IL-1β可能是OC的潜在诊断生物标志物。其中,TNF-α,IL-6和IL-8在OC的诊断中具有很高的准确性。然而,需要进一步的研究来消除其他混杂因素,需要更标准化的程序和大规模的研究来支持唾液检测的临床应用。
BACKGROUND: Oral cancer (OC) is a common malignancy in clinical practice. Saliva testing is a convenient and noninvasive early diagnostic technique for OC. Several salivary cytokines have been identified as potential biomarkers for OC, including IL-8, IL-6, TNF-α, IL-1β, and IL-10. Nonetheless, the optimal cytokine for OC diagnosis remains inconclusive and highly contentious.
METHODS: PubMed, Embase, Web of Science, and Cochrane Library databases were comprehensively retrieved to collect all case-control studies on OC. A meta-analysis was performed to compare the levels of salivary IL-8, IL-6, IL-10, TNF-α, and IL-1β in OC patients and healthy controls. Network meta-analysis (NMA) was carried out to probe into the accuracy of these salivary cytokines in diagnosing OC.
RESULTS: This analysis included 40 studies, encompassing 1280 individuals with OC and 1254 healthy controls. Significantly higher levels of salivary IL-8, IL-6, TNF-α, IL-1β, and IL-10 were observed in patients with OC in comparison to healthy controls. The results of NMA showed that TNF-α had the highest diagnostic accuracy for OC, with a sensitivity of 79% and a specificity of 92%, followed by IL-6 (sensitivity: 75%, specificity: 86%) and IL-8 (sensitivity: 80%, specificity: 80%).
CONCLUSIONS: This study suggests that IL-8, IL-6, IL-10, TNF-α, and IL-1β may be potential diagnostic biomarkers for OC. Among them, TNF-α, IL-6, and IL-8 are highly accurate in the diagnosis of OC. Nevertheless, further studies that eliminate other confounding factors are warranted, and more standardized procedures and large-scale studies are needed to support the clinical use of saliva testing.