本研究为鳞翅目害虫的靶向防治提供了一种有前途的核型多角体病毒(NPV)的控释形式。然而,由于NPV对UV失活的敏感性,其应用受到限制。这项研究调查了海藻酸钙(CA)包裹的SeMNPV闭塞体(OBs)微胶囊的抗紫外线性能,以及壳聚糖(CS)对CA的改性的影响,乳清蛋白(WP),和聚多巴胺(PDA)。这些胶囊用于递送,在碱性pH条件下的受控释放方式的病毒体。结构的表征,形态学,颗粒大小,封装效率,接触角,杀虫活性,进行了微胶囊的抗紫外线和体外释放。改性后的微胶囊具有较好的球形度,表面没有SeMNPVOB。包封率为84.76±0.59%。PDA@CA-NPV的润湿性最高,接触角为74.51±0.53°。CA-NPV的50%致死浓度值(LC50),CS@CA-NPV,WP@CA-NPV和PDA@CA-NPV分别是单独SeMNPVOB的11.5、10.7、10.5和1.2倍。改性后的微胶囊均提高了病毒的抗紫外线性能,PDA@CA-NPV是最耐紫外线的。使用qPCR,据观察,在碱性条件下,从PDA@CA-NPV释放了大量病毒粒子,CA-NPV和SeMNPVOBs。碱性条件下的微囊化病毒不会改变病毒体的释放模式。
This
study provides a promising controlled release form of nuclear polyhedrosis virus (NPV) for targeted control of lepidopteran pests. However, the application of NPV is limited due to its sensitivity to UV inactivation. This
study investigated the anti-UV properties of microcapsules of SeMNPV occlusion bodies (OBs) encapsulated by calcium alginate (CA), and also the influence of the modification of CA by chitosan (CS), whey protein (WP), and polydopamine (PDA). These capsules were used to deliver, in a controlled release manner virions under alkaline pH conditions. Characterization of the structure, morphology, particle size, encapsulation efficiency, contact angle, insecticidal activity, UV resistance and in vitro release of the microcapsules was conducted. The modified microcapsules had better sphericity, and were devoid of SeMNPV OBs on the surface. The encapsulation rate was 84.76 ± 0.59%. PDA@CA-NPV had the highest wettability and the contact angle was 74.51 ± 0.53°. The 50% lethal concentration values (LC50) of CA-NPV, CS@CA-NPV, WP@CA-NPV and PDA@CA-NPV were 11.5, 10.7, 10.5 and 1.2 times that of SeMNPV OBs alone. The modified microcapsules all improved the anti-UV performance of the virus, and PDA@CA-NPV was the most UV-resistant. Using qPCR, it was observed that under alkaline conditions, a large number of virions were released from PDA@CA-NPV, CA-NPV and SeMNPV OBs. Microencapsulated virus under alkaline conditions did not change the release pattern of virions.