背景：最近的全基因组关联研究发现，NCANrs2228603多态性与非酒精性脂肪性肝病（NAFLD）主要存在于欧洲血统的受试者中。虽然尚未进行研究来证明NCANrs2228603与中国汉族成年人NAFLD之间的关系。这项研究的目的是调查NCANrs2228603是否与中国人群的NAFLD相关。
方法：对182例NAFLD患者和195例健康对照者进行基因NCANrs2228603的基因分型。根据聚合酶链反应分析（PCR）检测NCAN的表达，并通过生物学技术进行血脂检测。
结果：NAFLD组与对照组之间NCANrs2228603的基因型和等位基因频率差异无统计学意义（P>0.05）。具有NCANrs2228603CT基因型的受试者显示较高水平的碱性磷酸酶（AKP）（P=0.017）和较高的高密度脂蛋白（HDL）（P<0.05）。
结论：我们的研究首次发现NCANrs2228603基因不是中国人群NAFLD发病率的危险因素。我们还发现T变体在保护肝脏中具有较高水平的HDL和赋予肝脏损伤风险的双重和相反的作用。
背景：中国临床试验注册.gov标识符：ChiCTR-ROC-15006447。
BACKGROUND: Recently genome-wide association studies identified that NCAN rs2228603 polymorphism was associated with non-alcoholic fatty liver disease (NAFLD) mainly in subjects of European ancestry. While no research have been conducted to demonstrate the relationship between NCAN rs2228603 and NAFLD in Chinese Han adults. The aim of this study was to investigate whether NCAN rs2228603 is associated with NAFLD in Chinese population.
METHODS: Gene NCAN rs2228603 was genotyped in 182 patients with NAFLD and 195 healthy controls. The expression of NCAN was tested according to polymerase chain reaction analysis (PCR) and serum lipids were performed by biology techniques.
RESULTS: No significant difference was found in genotype and allele frequencies of NCAN rs2228603 between the NAFLD group and the controls (P > 0.05). Subjects with the NCAN rs2228603 CT genotype showed a higher level of alkaline phosphatase (AKP) (P = 0.017) and a higher high-density lipoprotein (HDL) (P < 0.05).
CONCLUSIONS: Our study for the first time identified that the gene NCAN rs2228603 is not a risk factor for the incidence of NAFLD in Chinese population. Also we found the dual and opposite role of T variant in protecting liver with a higher level of HDL and conferring risk for liver damage with a higher level of AKP.
BACKGROUND: Chinese Clinical Trial Register.gov Identifier: ChiCTR-ROC-15006447 .
