新孢子虫病,一种由原生动物新孢子虫引起的传染病,与全世界养牛的经济损失有关。然而,以前的研究没有提供任何关于新孢子虫病血清学状态与生殖参数改变之间关联的证据。因此,这项研究的目的是确定是否与生殖疾病有关,并评估感染的可能危险因素。202头奶牛的血液样本,有生殖障碍病史的51人(病例组)和无生殖障碍病史的151人(对照组),是从巴西不同的农场收集的。对所有农民进行了流行病学问卷调查。对血清样品进行间接荧光抗体测试以检测针对寄生虫的抗体。总的来说,28.22%(57/202)的母牛血清呈阳性:病例组为47.06%(24/51),对照组为21.85%(33/151)。通过逻辑回归,年龄≥48个月的母牛和有流产史的母牛血清阳性的可能性分别为4.8倍(95%置信区间[CI]=1.91-12.05;p=0.001)和2.3倍(95%CI=1.06-5.1;p=0.034),分别。此外,我们的研究结果表明,在管理条件差的巴西奶牛中,N.caninum血清阳性与流产之间存在关联,同时也存在繁殖障碍的N.caninum血清阳性危险因素.
Neosporosis, an infectious disease caused by the protozoan
Neospora caninum, has been associated with economic losses in cattle rearing worldwide. However, previous studies have not presented any evidence regarding the association between serological status of neosporosis and alteration of the reproductive parameters. Thus, this study aimed to determine whether N. caninum is associated with reproductive disorders and to evaluate the possible risk factors of the infection. Blood samples from 202 dairy cows, 51 with a history of reproductive disorders (
case group) and 151 without (control group), were collected from different farms in Brazil. Epidemiological questionnaires were conducted with all the farmers. Serum samples were subjected to an indirect fluorescent antibody test to detect antibodies against the parasite. In total, 28.22% (57/202) of the cows were seropositive: 47.06% (24/51) from the
case group and 21.85% (33/151) from the control group. By logistic regression, cows aged ≥48 months and cows with history of abortion were 4.8 (95% confidence interval [CI] = 1.91-12.05; p = 0.001) and 2.3 (95% CI = 1.06-5.1; p = 0.034) times more likely to be seropositive, respectively. Furthermore, our results show an association between N. caninum seropositivity and abortion in dairy cows from Brazil with poor management conditions and N. caninum seropositivity risk factors for reproductive disorders.