从自体干细胞再生器官的令人兴奋的潜力近在眼前,和成人真皮干细胞(DSC)是特别有吸引力的,因为皮肤收集的容易和相对最小的侵入性。大量的报道已经描述了DSCs及其从间充质再生组织的潜力,外胚层,和内胚层谱系;然而,这些干细胞在各种皮肤类型中的确切生态位及其抗原表面组成尚未明确定义。DSC的多谱系潜力似乎相似,尽管分离和体外繁殖方法差异很大。尽管潜力巨大,仅有限量的组织和器官再生的临床应用已经从DSC开发出来。这篇综述总结了有关DSC的生态位及其表达的特定标记的文献。讨论了生态位的概念以及沿着特定谱系驻留在其中的细胞的分化能力。此外,考虑了广泛使用的证明谱系分化的方法的优缺点。此外,讨论了使用DSC的组织工程和再生领域的安全性考虑和最新进展。这篇综述总结了如何使用DSC前瞻性地进行组织和器官再生工程的思考。我们的期望是,本综述中强调的要点的实施将导致再生医学和组织工程领域的重大进步。
结论:自体真皮来源的干细胞在再生医学和组织工程领域引起了极大的兴奋和努力。这篇评论的实质性影响在于其对现有文献的批判性报道,并提供有关利基的见解,特点,和从人真皮来源的干细胞的分离方法。此外,它提供了使用人源性真皮干细胞的当前最先进的再生方法的分析,考虑到目前的指导方针,以帮助转化为治疗用途。
The exciting potential for regenerating organs from autologous stem cells is on the near horizon, and adult dermis stem cells (DSCs) are particularly appealing because of the ease and relative minimal invasiveness of skin collection. A substantial number of reports have described DSCs and their potential for regenerating tissues from mesenchymal, ectodermal, and endodermal lineages; however, the exact niches of these stem cells in various skin types and their antigenic surface makeup are not yet clearly defined. The multilineage potential of DSCs appears to be similar, despite great variability in isolation and in vitro propagation methods. Despite this great potential, only limited amounts of tissues and clinical applications for organ regeneration have been developed from DSCs. This
review summarizes the literature on DSCs regarding their niches and the specific markers they express. The concept of the niches and the differentiation capacity of cells residing in them along particular lineages is discussed. Furthermore, the advantages and disadvantages of widely used methods to demonstrate lineage differentiation are considered. In addition, safety considerations and the most recent advancements in the field of tissue engineering and regeneration using DSCs are discussed. This
review concludes with thoughts on how to prospectively approach engineering of tissues and organ regeneration using DSCs. Our expectation is that implementation of the major points highlighted in this
review will lead to major advancements in the fields of regenerative medicine and tissue engineering.
CONCLUSIONS: Autologous dermis-derived stem cells are generating great excitement and efforts in the field of regenerative medicine and tissue engineering. The substantial impact of this
review lies in its critical coverage of the available literature and in providing insight regarding niches, characteristics, and isolation methods of stem cells derived from the human dermis. Furthermore, it provides analysis of the current state-of-the-art regenerative approaches using human-derived dermal stem cells, with consideration of current guidelines, to assist translation toward therapeutic use.