含有自然资源的特定产品可以促进全口义齿卫生的创新。
目的:对含有BowdichiavirgilioidesKunth(BvK)精油的实验洁牙剂进行体外评估,:桉树(Ec),互叶白千层(Ma)和松果松(Ps)占1%。
方法:评估的变量是感官和理化特性,磨损性(机械刷机)模拟2.5年,和微生物负荷(菌落形成单位-CFU/mL),多物种生物膜(变异链球菌:Sm,金黄色葡萄球菌:Sa,白色念珠菌:Ca和光滑念珠菌:Cg)。热聚合丙烯酸树脂样品(n=256)(n=96个磨蚀样品,对于微生物负荷计数,n=72,对于生物膜代谢活性,n=72,对于细胞活力和总生物膜定量,n=16)将形成的生物膜分为八组,用牙刷和蒸馏水(NC:阴性对照)手动刷牙(20秒),三环(PC:阳性对照),安慰剂(Pl),BvK,Co,Ec,马或PS。刷牙后,样品用PBS洗涤并浸入LetheenBroth培养基中,并将悬浮液播种在固体特定培养基中。通过描述性分析呈现感官特征。密度值,pH值,稠度和粘度列于表中。在广义线性模型中使用Wald检验对数据进行分析,接着是Kruskal-Wallis测试,Dunn检验(质量变化)和Bonferroni检验(UFC和XTT)。使用广义估计方程中的Wald检验和Bonferroni检验来分析细胞活力。
结果:所有洁齿剂均显示出稳定的感官特性和足够的理化性质。CN,Ec,PS,Pl和PC显示低磨损性。在微生物负荷方面,各组之间存在显着差异(p<0.001),代谢活性和生物膜活力。
结论:结论是BvK,Ec和Ps洁牙剂可用于清洁全口义齿,因为它们对生物膜具有抗菌活性。含有virgilioidesKunth的Bowdichia洁牙剂具有中等磨损性,应谨慎使用。
Specific products containing natural resources can contribute to the innovation of complete denture hygiene.
OBJECTIVE: To conduct an in vitro evaluation of experimental dentifrices containing essential oils of Bowdichia virgilioides Kunth (BvK), Copaifera officinalis (Co), Eucalyptus citriodora (Ec), Melaleuca alternifolia (Ma) and Pinus strobus (Ps) at 1%.
METHODS: The variables evaluated were organoleptic and physicochemical characteristics, abrasiveness (mechanical brushing machine) simulating 2.5 years, and microbial load (Colony Forming Units - CFU/mL), metabolic activity (XTT assay) and cell viability (Live/Dead® BacLight™ kit) of the multispecies biofilm (Streptococcus mutans: Sm, Staphylococcus aureus: Sa, Candida albicans: Ca and Candida glabrata: Cg). Specimens of heat-polymerized acrylic resins (n=256) (n=96 specimens for abrasiveness, n=72 for microbial load count, n=72 for biofilm metabolic activity, n=16 for cell viability and total biofilm quantification) with formed biofilm were divided into eight groups for manual brushing (20 seconds) with a dental brush and distilled water (NC: negative control), Trihydral (PC: positive control), placebo (Pl), BvK, Co, Ec, Ma or Ps. After brushing, the specimens were washed with PBS and immersed in Letheen Broth medium, and the suspension was sown in solid specific medium. The organoleptic characteristics were presented by descriptive analysis. The values of density, pH, consistency and viscosity were presented in a table. The data were analyzed with the Wald test in a generalized linear model, followed by the Kruskal-Wallis test, Dunn\'s test (mass change) and the Bonferroni test (UFC and XTT). The Wald test in Generalized Estimating Equations and the Bonferroni test were used to analyze cell viability.
RESULTS: All dentifrices showed stable organoleptic characteristics and adequate physicochemical properties. CN, Ec, Ps, Pl and PC showed low abrasiveness. There was a significant difference between the groups (p<0.001) for microbial load, metabolic activity and biofilm viability.
CONCLUSIONS: It was concluded that the BvK, Ec and Ps dentifrices are useful for cleaning complete dentures, as they have antimicrobial activity against biofilm. The dentifrices containing Bowdichia virgilioides Kunth showed medium abrasiveness and should be used with caution.