水和冷冻保护剂(CPA)分子的细胞膜渗透系数值是开发描述通过细胞膜传质过程的物理数学模型以预测最佳细胞冷却速率的必要特征。我们对小鼠卵母细胞膜对水分子的渗透系数进行了比较分析,乙二醇(EG),丙烷-1,2-二醇(1,2-PD)和二甲基亚砜(Me2SO),通过应用经典的Kedem-Katchalsky模型确定,只考虑非电解质分子(水和CPA)通过膜的渗透,和我们开发的模型,其中考虑了离子的跨膜转移以及跨膜电势的相关变化。我们表明,基于开发的修改模型的计算为水和CPA分子提供了较低的卵母细胞膜渗透系数值。重要的是,通过我们修改的模型获得的水分子的渗透系数不取决于冷冻保护剂的类型,而经典模型在我们的研究和其他作者的作品中的应用总是在具有不同冷冻保护剂的溶液中给出这些系数的不同值。我们修改的模型还可以确定在跨膜传质条件下细胞的跨膜电势的动力学以及受电势变化影响的膜持续时间,这是一个可以直接影响细胞活力的参数。
Values of cell membranes permeability coefficients for water and molecules of cryoprotective agents (CPAs) are the necessary characteristics for developing physical-mathematical models describing mass transfer processes through cell membranes in order to predict optimal cell cooling rates. We carried out a comparative analysis of the permeability coefficients of mouse oocyte membranes for molecules of water, ethylene glycol (EG), propane-1,2-diol (1,2-PD) and dimethyl sulfoxide (Me2SO), determined by applying the classical Kedem-Katchalsky model, which considers only the penetration of non-electrolyte molecules (water and CPA) through the membrane, and the model developed by us, which takes into account the transmembrane transfer of ions and the associated changes in the transmembrane electric potential. We shown that calculations based on the developed modified model provide lower values of the permeability coefficients of the oocyte membrane for water and CPA molecules. What is important that the obtained by our modified model permeability coefficients for water molecules do not depend on the type of cryoprotectant, while the application of the classical model both in our studies and works of other authors always gave different values of these coefficients in solutions with different cryoprotectants. Our modified model also makes it possible to determine the dynamics of the transmembrane electric potential of the cell under the conditions of transmembrane mass transfer and the duration of the membrane being influenced by the changes in electric potential, that is a parameter that can directly affect the viability of cells.