慢性乙型肝炎感染(CHB)是全球肝细胞癌(HCC)发展的主要危险因素,并继续构成重大的全球健康挑战。加味银陈蒿汤(JWYCH)是银陈蒿汤(YCHD)的改良版,广泛用于治疗肝病,包括黄疸性肝炎,胆石症,和肝性腹水.然而,JWYCH对CHB的有效性和潜在机制尚不清楚。本研究旨在探讨JWYCH对CHB的影响,并通过网络药理学和代谢组学探讨其潜在机制。通过流体动力学注射(HDI)给C57BL/6小鼠施用rAAV-HBV1.3以建立CHB模型。将感染的小鼠口服施用JWYCH4周。HBsAg,HBeAg,HBVDNA,血清肝功能指标,和组织病理学检测。此外,网络药理学用于研究潜在的靶标,而非靶向代谢组学分析用于探索CHB小鼠JWYCH的肝脏代谢变化,并鉴定相关的生物标志物和代谢途径。JWYCH能够降低HBeAg水平并改善CHB小鼠的肝脏病理变化。此外,代谢组学分析表明,JWYCH可以影响105种代谢物,包括哌啶酸,α-萜品烯,腺苷,和L-苯丙氨酸,在其他人中。胆汁酸代谢,花生四烯酸代谢,和视黄醇代谢被认为是CHB中JWYCH的潜在靶标。总之,JWYCH显示对CHB小鼠模型的肝保护作用,提示CHB的潜在替代治疗策略。JWYCH的作用主要与调节胆汁酸的代谢有关,花生四烯酸,还有视黄醇.这些差异丰富的代谢物可以作为CHB的潜在生物标志物和治疗靶标。
Chronic hepatitis B infection (CHB) is a major risk factor for the development of hepatocellular carcinoma (HCC) globally and continues to pose a significant global health challenge. Jiawei Yinchenhao decoction (JWYCH) is a modified version of Yinchenhao decoction (YCHD), which is widely used to treat liver diseases including icteric hepatitis, cholelithiasis, and hepatic ascites. However, the effectiveness and underlying mechanism of JWYCH on CHB are still unclear. This
study aimed to investigate the impact of JWYCH on CHB and explore the underlying mechanism via network pharmacology and
metabolomics. C57BL/6 mice were administered rAAV-HBV1.3 via hydrodynamic injection (HDI) to establish the CHB model. The infected mice were orally administered JWYCH for 4 weeks. HBsAg, HBeAg, HBV DNA, the serum liver function index, and histopathology were detected. In addition, network pharmacology was used to investigate potential targets, whereas untargeted
metabolomics analysis was employed to explore the hepatic metabolic changes in JWYCH in CHB mice and identify relevant biomarkers and metabolic pathways. JWYCH was able to reduce HBeAg levels and improve liver pathological changes in mice with CHB. Additionally,
metabolomics analysis indicated that JWYCH can influence 105 metabolites, including pipecolic acid, alpha-terpinene, adenosine, and L-phenylalanine, among others. Bile acid metabolism, arachidonic acid metabolism, and retinol metabolism are suggested to be potential targets of JWYCH in CHB. In conclusion, JWYCH demonstrated a hepatoprotective effect on a mouse model of CHB, suggesting a potential alternative therapeutic strategy for CHB. The effect of JWYCH is associated mainly with regulating the metabolism of bile acid, arachidonic acid, and retinol. These differentially abundant metabolites may serve as potential biomarkers and therapeutic targets for CHB.