丰霉素是脂肽家族的重要成员,在农业上有着广泛的应用,食物,医疗和化妆品行业。然而,低生产率和高成本严重阻碍了其商业应用。因此,许多研究已经致力于提高芬霉素的产量。本文对这些研究进行综述,旨在为今后的研究人员提供参考和指导。本文首先概述了通过非核糖体肽合成酶(NRPS)合成芬霉素的机理,然后深入研究了近年来提高fengycin产量的策略。这些策略主要包括发酵优化和代谢工程,代谢工程包括增加前体供应,调节因子的应用,启动子工程,基因组工程(基因组改组和基因组尺度代谢网络模型)的应用。最后,最后,我们对fengycin的生产进行了展望。
Fengycin is an important member of the
lipopeptide family with a wide range of applications in the agricultural, food, medical and cosmetic industries. However, its commercial application is severely hindered by low productivity and high cost. Therefore, numerous studies have been devoted to improving the production of fengycin. We summarize these studies in this
review with the aim of providing a reference and guidance for future researchers. This
review begins with an overview of the synthesis mechanism of fengycin via the non-ribosomal peptide synthetases (NRPS), and then delves into the strategies for improving the fengycin production in recent years. These strategies mainly include fermentation optimization and metabolic engineering, and the metabolic engineering encompasses enhancement of precursor supply, application of regulatory factors, promoter engineering, and application of genome-engineering (genome shuffling and genome-scale metabolic network model). Finally, we conclude this
review with a prospect of fengycin production.