目的:研究已经确定,具有遗传定义的乳糖酶非持久性的人的乳制品摄入量较低,这可能导致各种非传染性疾病的风险增加。此外,乳糖酶的非持久性本身与胰岛素抵抗有关。然而,发展中国家乳糖酶非持久性状态和乳制品摄入量的数据很少。因此,我们旨在定义1)泰国人群中糖尿病和非糖尿病个体中乳糖酶非持久性的患病率,以及2)乳糖酶非持久性之间的联系,牛奶消费,和糖尿病的风险。
方法:我们对全国健康检查调查的参与者进行了病例对照研究。从血液中分离出LCT-13910C>T(rs4988235)多态性的DNA,并使用Bio-radc1000触摸热循环仪和MALDI-TOF质谱MassARRAYTyperv4.0(AgenaBioscience,圣地亚哥,CA,美国)在医学基因组学中心,医学院Ramathibodi医院。病例为先前诊断为糖尿病或空腹血糖≥126mg/dL(n=1,756)与控件(n=2,380)。
结果:我们包括4,136名参与者,62%女性,98.8%>30岁。纯合CC基因型(即,乳糖酶非持久性)占98.6%,只有1.4%携带杂合CT。大多数(76%)每月食用牛奶<1份。具有CC或CT基因型的参与者的牛奶摄入量和糖尿病风险相当。男性,老年人,教育程度较低的人每月至少食用一份牛奶的机会较低。除了各种基线变量,我们发现,较高的牛奶摄入量与较低的DM风险相关(P=0.01)。
结论:泰国人群中乳糖酶非持久性的患病率非常高。未发现与乳糖酶非持久性状态相关的牛奶消费频率存在显着差异。然而,较高的牛奶摄入量与较低的糖尿病风险相关。
Studies have determined that people with genetically defined lactase non-persistence have lower dairy intake that may lead to an increase risk of various non-communicable diseases. Furthermore, lactase non-persistence itself has been associated with insulin resistance. However, data on lactase non-persistence status and dairy intake in developing countries are sparse. We therefore aimed to define 1) the prevalence of lactase non-persistence among individuals with diabetes and non-diabetes in Thai population and 2) the links between lactase non-persistence, milk consumption, and risk of diabetes mellitus.
We conducted a
case-control study from participants of the National Health Examination Survey. DNA was isolated from the blood for LCT -13910C>T (rs4988235) polymorphism and processed using the Bio-rad c1000 touch thermal cycler and MALDI-TOF Mass Spectrometry MassARRAY Typer v4.0 (Agena Bioscience, San Diego, CA, USA) at the Center for Medical Genomics, Faculty of Medicine Ramathibodi Hospital. Cases were participants with previously diagnosed diabetes mellitus or fasting plasma glucose ≥126 mg/dL (n = 1,756) vs. the controls (n = 2,380).
We included 4,136 participants, 62% female, and 98.8% were > 30 years old. Homozygous CC genotype (i.e., lactase non-persistence) was noted in 98.6% and only 1.4% carried heterozygous CT. Most (76%) consumed milk <1 portion/month. Participants with either CC or CT genotype had comparable milk consumption and the risk of diabetes mellitus. Males, older adults, and lower education had a lower chance of consuming milk at least one portion per month. Besides various baseline variables, we found that higher milk consumption was associated with a lower DM risk (P = .01).
The prevalence of lactase non-persistence in Thai population is very high. A significant difference in milk consumption frequency in relation to the lactase non-persistence status was not found. However, higher milk consumption is associated with a lower risk of diabetes mellitus.