约翰氏病(JD)是由鸟分枝杆菌亚种副结核(MAP)引起的反刍动物的慢性肠道感染。JD感染在山羊中比牛更难诊断,因为MAP可以隐匿地影响小反刍动物。很少有报道对韩国黑山羊的病例进行病理和分子诊断。这里,我们提供了两只MAP感染山羊的结果。病例1被归类为临床意义(IV期),具有严重的临床体征和高抗体滴度(S/P比,158.9%)。病例2处于亚临床阶段(II期);然而,山羊突然死亡,没有任何临床症状(S/P比,70.9%)。来自病例1的器官组织和粪便的DNA显示出MAP的强阳性PCR结果,而案例2仅在粪便样品中表现出非常弱的反应。此外,使用PCR-REA方法将来自两个病例的粪便DNA基因分型为C型MAP。胃肠道器官组织(空肠,回肠,结肠,和肠系膜淋巴结)来自病例1显示中度至重度病变,观察到抗酸杆菌。相比之下,病例2显示完整至轻度的病理病变,在结肠中检测到抗酸杆菌,肠系膜淋巴结,还有肝脏.此外,病例2与假结核棒状杆菌共感染,导致了干酪样淋巴结炎.本案例研究提供了有关JD感染的韩国黑山羊的病理和分子特征的有价值的信息。结果突出了临床和亚临床感染山羊之间病理病变的差异,这有助于兽医制定更好的策略来控制山羊养殖场的MAP。
Johne\'s disease (JD) is a chronic enteric infection in ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). JD infection is more difficult to diagnose in goats than cattle because MAP can insidiously affect small ruminants. Few
reports have used pathological and molecular diagnosis for cases in Korean black goats. Here, we present our results from two MAP-infected goats. Case 1 was categorized as clinically significant (stage IV), with severe clinical signs and a high antibody titer (S/P ratio, 158.9%).
Case 2 was in the subclinical stage (stage II); however, the goat suddenly died without any clinical signs (S/P ratio, 70.9%). DNA from the organ tissues and feces from
Case 1 showed a strong positive PCR result for MAP, whereas
Case 2 only exhibited a very weak reaction in the fecal sample. Moreover, fecal DNA from both cases was genotyped as C-type MAP using the PCR-REA method. Gastrointestinal organ tissues (jejunum, ileum, colon, and mesenteric lymph nodes) from
Case 1 showed moderate-to-severe lesions, and acid-fast bacilli were observed. In contrast, Case 2 showed intact-to-mild pathological lesions, and acid-fast bacilli were detected in the colon, mesenteric lymph nodes, and liver. In addition, Case 2 was co-infected with Corynebacterium pseudotuberculosis, which caused caseous lymphadenitis. This case study provides valuable information regarding the pathological and molecular characteristics of JD-infected Korean black goats. The results highlighted the differences in pathological lesions between clinically and subclinically infected goats, which help veterinarians to develop better strategies to control MAP in goat farms.