怀孕的成功在很大程度上取决于平衡的免疫稳态。在非孕妇和孕妇的上生殖道中检测到细菌成分引起了人们对其在生殖健康中可能的有益作用的疑问。允许细菌存在与怀孕建立协调的当地条件仍然未知。在子宫内膜和胎盘样本中描述的细菌种类中,发现了具核梭杆菌。已经观察到,F.nucleatum可以诱导结肠癌的肿瘤发生,一个与胚胎植入共有几个特征的过程。我们建议低浓度的F.核仁可以改善滋养细胞的功能,而不会产生破坏性反应。将失活的有核F.和大肠杆菌与滋养细胞系HTR8/SVneo一起孵育,BeWo,还有JEG-3.活力,扩散,迁徙能力,侵袭性和趋化因子的分泌,评估其他细胞因子和基质金属蛋白酶.F.核仁的存在显著诱导HTR8/SVneo侵袭,伴随着可溶性介质(CXCL1,IL-6和IL-8)和金属蛋白酶(MMP-2和MMP-9)的分泌。然而,随着F.核仁浓度的增加,这些并没有提高侵袭性,阻碍移民,降低细胞活力并诱导细胞周期的改变。通过添加TLR4阻断抗体,部分核仁F.对细胞因子释放的作用得以恢复。其他作用与E-钙黏着蛋白在测试的不同细胞系上的表达水平相关。少量的F.核仁促进HTR8/SVneo细胞的侵袭并诱导重要介质的分泌以建立妊娠。某些作用与LPS无关,并且与E-cadherin对滋养细胞的表达有关。
Pregnancy success depends greatly on a balanced immune homeostasis. The detection of bacterial components in the upper reproductive tract in non-pregnant and pregnant women raised questions on its possible beneficial role in reproductive health. The local conditions that allow the presence of bacteria to harmonize with the establishment of pregnancy are still unknown. Among the described bacterial species in endometrial and placental samples, Fusobacterium nucleatum was found. It has been observed that F. nucleatum can induce tumorigenesis in colon carcinoma, a process that shares several features with embryo implantation. We propose that low concentrations of F. nucleatum may improve trophoblast function without exerting destructive responses. Inactivated F. nucleatum and E. coli were incubated with the trophoblastic cell lines HTR8/SVneo, BeWo, and JEG-3. Viability, proliferation, migratory capacity, invasiveness and the secretion of chemokines, other cytokines and matrix metalloproteinases were assessed. The presence of F. nucleatum significantly induced HTR8/SVneo invasion, accompanied by the secretion of soluble mediators (CXCL1, IL-6 and IL-8) and metalloproteinases (MMP-2 and MMP-9). However, as concentrations of F. nucleatum increased, these did not improve invasiveness, hindered migration, reduced cell viability and induced alterations in the cell cycle. Part of the F. nucleatum effects on cytokine release were reverted with the addition of a TLR4 blocking antibody. Other effects correlated with the level of expression of E-cadherin on the different cell lines tested. Low amounts of F. nucleatum promote invasion of HTR8/SVneo cells and induce the secretion of important mediators for pregnancy establishment. Some effects were independent of LPS and correlated with the expression of E-cadherin on trophoblasts.