本研究旨在探讨消癌平注射液联合替吉奥抑制人GC细胞侵袭转移的作用及机制。
BGC-823和MGC-803细胞体外培养,MTT法评价各处理对BGC-823和MGC-803细胞的细胞毒性和增殖的影响。采用细胞粘附试验和Transwell法检测消癌平注射液联合S-1对BGC-823和MGC-803细胞转移能力的影响。VEGF的表达,通过蛋白质印迹法检测与上皮-间充质转化(EMT)相关的金属蛋白酶(MMPs)和蛋白质。同时,在裸鼠中建立肿瘤模型,并研究了XAP联合S-1对体内BGC-823细胞的影响。
与单一药物组相比,XAP与S-1的组合可提高抑制率(P<0.05)。黏附试验显示,联合组能显著抑制BGC-823和MGC-803细胞的黏附(P<0.05)。XAP与S-1的组合降低了人GCBGC-823和MGC-803细胞的迁移和侵袭潜力。Westernblotting显示VEGF的表达,与XAP或S-1单独组的表达值相比,联合组的MMP-9,Ncadherin和波形蛋白降低,而E-cadherin表达增加(P<0.05)。在体内,我们发现,与单独使用XAP或S-1相比,XAP联合S-1对肿瘤生长具有显著的抑制作用.免疫组织化学显示,XAP与S-1联合能够提高E-cadherin的水平,并下调N-cadherin和波形蛋白。
XAP与S-1的组合可以增强单一药物对增殖的抑制作用,侵袭和转移。其机制可能与VEGF和MMP-9蛋白表达降低及对EMT的影响有关。
This
study aimed to determine the effect and mechanism of Xiaoaiping (XAP) injection combined with S-1 in inhibiting the invasion and metastasis of human GC cells.
BGC-823 and MGC-803 cells were incubated in vitro, and the effects of treatment on the cytotoxicity and proliferation of BGC-823 and MGC-803 cells were evaluated by MTT assay. Cell adhesion tests and Transwell assays were used to detect the effects of Xiaoaiping injection combined with S-1 on the metastatic ability of BGC-823 and MGC-803 cells. The expression of VEGF, Metalloproteinases (MMPs) and proteins related to the Epithelial-Mesenchymal Transition (EMT) were detected by Western blotting. Meanwhile, a tumour model was established in nude mice, and the effect of XAP combined with S-1 on BGC-823 cells in vivo was studied.
Compared with the single drug group, the combination of XAP with S-1 increased the inhibition rate (P<0.05). The adhesion test showed that the combination group significantly inhibited the adhesion of BGC-823 and MGC-803 cells (P<0.05). The combination of XAP with S-1 reduced the migration and invasion potential of human GC BGC-823 and MGC-803 cells. Western blotting showed that the expression of VEGF, MMP-9, Ncadherin and vimentin was decreased and E-cadherin expression was increased in the combination group compared with these expression values in either the XAP or S-1 alone group (P<0.05). In vivo, we found that XAP combined with S-1 had a significant inhibitory effect on the growth of tumours compared with XAP or S-1 alone. Immunohistochemistry showed that XAP combined with S-1 was able to enhance the levels of E-cadherin and downregulate N-cadherin and vimentin.
The combination of XAP with S-1 can enhance the inhibitory effect of a single drug on proliferation, invasion and metastasis. The mechanism may be related to the decrease in the expression of VEGF and MMP-9 proteins and the effect on EMT.