这项研究的目的是测量呼吸道感染期间婴儿白细胞中的干扰素γ(IFN-γ)和吲哚胺2,3-双加氧酶1(IDO1)值,并将其与健康的年龄相匹配的对照进行比较。
这是一个前景,2019-2020年进行的观察性病例对照研究。
这项研究在ReginaMargherita儿童医院进行,都灵,意大利。
这项研究包括63名婴儿,包括26例因呼吸道合胞病毒(RSV)感染而住院的细支气管炎患者和37例年龄匹配的对照。纳入标准包括患有细支气管炎的婴儿的阳性RSV测试。
我们收集外周血并用TaqMan实时PCR扩增测量IFN-γ和IDO1的信使RNA(mRNA)表达的相对定量。数据是在入院的第一天收集的。
26例RSV毛细支气管炎患者(53.8%为女性)入院时的平均年龄为85(9-346)天。他们出生时的平均胎龄为38周,平均出生体重为3100(2780-3730)g。与健康对照组相比,毛细支气管炎RSV患者的IFN-γ表达显着降低(p=0.0132)。然而,当测量其WCC中的IDO1mRNA表达值时,两组之间没有显着差异(p=0.0642)。
我们的研究结果没有阐明IDO1的表达是否与疾病的早期阶段或婴儿的年轻年龄有关。数据提供了证据表明,由于RSV,患有细支气管炎的婴儿IFN-γ显着降低,与年龄匹配的健康对照相比,但IDO1并没有什么不同。关注在婴儿期不同阶段感染RSV的受试者的新研究将有助于阐明IDO1表达是否与年龄有关。
The aim of this study was to measure interferon gamma (IFN-γ) and indoleamine 2,3-dioxygenase 1 (IDO1) values in the White blood cells of infants during respiratory tract infections and to compare these with healthy age-matched controls.
This was a prospective, observational
case-control study conducted in 2019-2020.
The study took place at Regina Margherita Children\'s Hospital, Turin, Italy.
The study comprised 63 infants, including 26 patients hospitalised for bronchiolitis due to a respiratory syncytial virus (RSV) infection and 37 age-matched controls. The inclusion criteria included a positive RSV test for an infant with bronchiolitis.
We collected peripheral blood and measured the relative quantification of messenger RNA (mRNA) expression of IFN-γ and IDO1 with TaqMan real-time PCR amplification. The data were collected on the first day of admission.
The mean age of the 26 patients with RSV bronchiolitis (53.8% female) was 85 (9-346) days when they were admitted to the hospital. Their mean gestational age at birth was 38 weeks and their mean birth weight was 3100 (2780-3730) g. The expression of IFN-γ was significantly reduced in patients with bronchiolitis RSV compared with healthy controls (p=0.0132). However, there was no significant difference between the two groups when the IDO1 mRNA expression values in their WCC were measured (p=0.0642).
Our findings did not clarify whether IDO1 expression was related to the early stage of the disease or to the young age of the infants. The data provide evidence that IFN-γ was significantly reduced in infants with bronchiolitis due to RSV, compared with age-matched healthy controls, but the IDO1 was not different. New investigations that focus on subjects infected with RSV at different stages of infancy would help to clarify whether IDO1 expression can be related to age.