背景:纳米酶,一类新的纳米材料,由于其出色的稳定性,已成为生物传感器设计中酶的有希望的替代品,负担能力,和准备的可用性。虽然纳米酶解决了天然酶的许多限制,他们仍然面临挑战,特别是在达到其天然对应物的催化活性水平。这表明需要增强基于纳米酶的生物传感器的灵敏度。通过调节纳米酶的大小可以显著提高其催化活性,形态学,和纳米材料的表面组成。
结果:在这项工作中,设计了一种中空核壳结构以增强纳米酶的催化活性。中空核壳结构材料由纳米酶核层组成,一个空心层,和MOF壳层。以经典的过氧化物酶如Fe3O4为例,一种新型纳米酶@MOF的开发,特别是p-Fe3O4@PDA@ZIF-67,展示了其在增强基于Fe3O4纳米酶的传感器灵敏度方面的应用。这种创新的纳米复合材料,MOF层设计用于吸附传感器的信号分子,以提高纳米酶催化反应产生的活性氧的利用率,中空层设计用于防止纳米酶的活性位点被MOF层覆盖。手稿强调了纳米复合材料在检测过氧化氢(H2O2)方面的卓越灵敏度,再加上高特异性和可重复性,即使在牛奶样品等复杂环境中。
■这项工作首次提出并证明了具有中空层结构的Fe3O4纳米酶@MOF旨在提高Fe3O4纳米酶的催化活性和基于Fe3O4纳米酶的传感器的灵敏度。这项研究标志着纳米酶技术的重大进步,展示结构创新在创造高性能方面的潜力,敏感,和稳定的生物传感器的各种应用。
BACKGROUND: Nanozymes, a new class of nanomaterials, have emerged as promising substitutes for enzymes in biosensor design due to their exceptional stability, affordability, and ready availability. While nanozymes address many limitations of natural enzymes, they still face challenges, particularly in achieving the catalytic activity levels of their natural counterparts. This indicates the need for enhancing the sensitivity of biosensors based on nanozymes. The catalytic activity of nanozyme can be significantly improved by regulating its size, morphology, and surface composition of nanomaterial.
RESULTS: In this work, a kind of hollow core-shell structure was designed to enhance the catalytic activity of nanozymes. The hollow core-shell structure material consists of a nanozymes core layer, a hollow layer, and a MOF shell layer. Taking the classic peroxidase like Fe3O4 as an example, the development of a novel nanozyme@MOF, specifically p-Fe3O4@PDA@ZIF-67, is detailed, showcasing its application in enhancing the sensitivity of sensors based on Fe3O4 nanozymes. This innovative nanocomposite, featuring that MOF layer was designed to adsorb the signal molecules of the sensor to improve the utilization rate of reactive oxygen species generated by the nanozymes catalyzed reactions and the hollow layer was designed to prevent the active sites of nanozymes from being cover by the MOF layer. The manuscript emphasizes the nanocomposite\'s remarkable sensitivity in detecting hydrogen peroxide (H2O2), coupled with high specificity and reproducibility, even in complex environments like milk samples.
UNASSIGNED: This work firstly proposed and proved that Fe3O4 nanozyme@MOF with hollow layer structure was designed to improve the catalytic activity of the Fe3O4 nanozyme and the sensitivity of the sensors based on Fe3O4 nanozyme. This research marks a significant advancement in nanozyme technology, demonstrating the potential of structural innovation in creating high-performance, sensitive, and stable biosensors for various applications.