背景:有研究表明,慢性低度炎症在多囊卵巢综合征(PCOS)的发病机制中起重要作用。根据以前的研究,目前尚不清楚哪些细胞因子会影响该综合征的发展,以及它们的增加是否与超重/肥胖的存在相关,或者是独立因素.我们研究的目的是确定PCOS女性与正常体重和超重亚组健康女性相比的慢性炎症参数。
方法:这项病例对照研究包括44例PCOS患者(19例体重指数(BMI)<25kg/m²的女性和25例BMI≥25kg/m²的女性)和45例没有PCOS症状的女性(22例BMI<25kg/m²的女性和23例BMI≥25kg/m²的女性)。使用免疫学多重测定HCYTA-60K-PX48(默克生命科学,LLC,德国)。
结果:细胞因子:白细胞介素-1受体拮抗剂(IL-1RA),IL-2,IL-6,IL-17E,IL-17A,与对照组相比,PCOS女性患者的IL-18和巨噬细胞炎性蛋白-1α(MIP-1α)增加,在瘦和超重/肥胖亚组(p<0.05)。此外,只有患有PCOS的瘦女性有更高水平的IL-1α,IL-4,IL-9,IL-12,IL-13,IL-15,肿瘤坏死因子(TNF-α)α和β,可溶性CD40及其配体(SCD40L),Fractalkine(FKN),单核细胞趋化蛋白3(MCP-3),和MIP-1β与对照组相比(p<0.05)。与对照组相比,PCOS女性(瘦和超重/肥胖)合并组的IL-22增加(p=0.012)。
结论:慢性低度炎症是影响PCOS发生的独立因素,并不依赖于超重/肥胖的存在。第一次,我们获得了PCOS女性中IL-9,MCP-3和MIP-1α等炎症参数增加的数据.
BACKGROUND: it has been suggested that chronic low-grade inflammation plays an important role in the pathogenesis of polycystic ovary syndrome (PCOS). According to previous studies, it remains unclear which cytokines influence the development of this syndrome and whether their increase is associated with the presence of excess weight/obesity or is an independent factor. The aim of our research was to determine the parameters of chronic inflammation in women with PCOS in comparison with healthy women in the normal weight and the overweight subgroups.
METHODS: This
case-control study included 44 patients with PCOS (19 women with a body mass index (BMI) < 25 kg/m² and 25 women with a BMI ≥ 25 kg/m²) and 45 women without symptoms of PCOS (22 women with a BMI < 25 kg/m² and 23 women with a BMI ≥ 25 kg/m²). Thirty-two cytokines were analyzed in the plasma of the participants using Immunology multiplex assay HCYTA-60K-PX48 (Merck Life Science, LLC, Germany).
RESULTS: Cytokines: interleukin-1 receptor antagonist (IL-1 RA), IL-2, IL-6, IL-17 E, IL-17 A, IL-18, and macrophage inflammatory protein-1 alpha (MIP-1 α) were increased in women with PCOS compared to controls, both in lean and overweight/obese subgroups (p < 0.05). Moreover, only lean women with PCOS had higher levels of IL-1 alpha, IL-4, IL-9, IL-12, IL-13, IL-15, tumor necrosis factor (TNF- α) alpha and beta, soluble CD40 and its ligand (SCD40L), fractalkine (FKN), monocyte-chemotactic protein 3 (MCP-3), and MIP-1 β compared to the control group (p < 0.05). IL-22 was increased in the combined group of women with PCOS (lean and overweight/obese) compared to the control group (p = 0.012).
CONCLUSIONS: Chronic low-grade inflammation is an independent factor affecting the occurrence of PCOS and does not depend on the presence of excess weight/obesity. For the first time, we obtained data on the increase in such inflammatory parameters as IL-9, MCP-3, and MIP-1α in women with PCOS.