几种不同的动物模型用于研究废用诱导的骨丢失。本系统综述旨在全面概述废用性骨质流失的动物模型,并提供每种独特动物模型的详细叙述合成。
从开始到2019年11月30日,系统地搜索了PubMed和Embase的废弃动物模型。此外,搜索了GoogleScholar和个人档案,以查找未在PubMed或Embase中索引的相关出版物。两名审稿人独立审查了标题和摘要,以便全文收录。使用预定义的提取方案提取数据以确保标准化。
1964年的标题和摘要进行了筛选,其中包括653篇全文文章。用于建立废弃模型的最常见动物种类是大鼠(59%)和小鼠(30%)。在大多数研究中使用的雄性(53%)和转基因动物占7%。确定了十二种不同的诱导废弃的方法。最常用的方法是后肢卸载(44%),神经切除术(15%),绷带和矫形器(15%),和肉毒杆菌毒素(9%)。中位废用时间为21天(四分位数:14天,36天),每组被废弃的动物数量中位数为10(四分位数:7、14)。43%的研究报告了随机分组。少于5%的研究通过样本量计算来证明每组动物的数量是合理的,以确保足够的统计能力。
存在多种废用性骨丢失的动物模型,已经成功地研究了几种动物。废用引起的骨丢失的复杂性保证了严格的研究设计。本系统综述强调了动物废弃研究和报告标准化的必要性。
Several different animal models are used to study disuse-induced bone loss. This systematic
review aims to give a comprehensive overview of the animal models of disuse-induced bone loss and provide a detailed narrative synthesis of each unique animal model.
PubMed and Embase were systematically searched for animal models of disuse from inception to November 30, 2019. In addition, Google Scholar and personal file archives were searched for relevant publications not indexed in PubMed or Embase. Two reviewers independently reviewed titles and abstracts for full-text inclusion. Data were extracted using a predefined extraction scheme to ensure standardization.
1964 titles and abstracts were screened of which 653 full-text articles were included. The most common animal species used to model disuse were rats (59%) and mice (30%). Males (53%) where used in the majority of the studies and genetically modified animals accounted for 7%. Twelve different methods to induce disuse were identified. The most frequently used methods were hindlimb unloading (44%), neurectomy (15%), bandages and orthoses (15%), and botulinum toxin (9%). The median time of disuse was 21 days (quartiles: 14 days, 36 days) and the median number of animals per group subjected to disuse was 10 (quartiles: 7, 14). Random group allocation was reported in 43% of the studies. Fewer than 5% of the studies justified the number of animals per group by a sample size calculation to ensure adequate statistical power.
Multiple animal models of disuse-induced bone loss exist, and several species of animals have successfully been studied. The complexity of disuse-induced bone loss warrants rigid research study designs. This systematic
review emphasized the need for standardization of animal disuse research and reporting.