■脓毒症可破坏免疫调节并经常导致急性呼吸窘迫综合征(ARDS)。雷莫唑仑,一种速效催眠药物,与其他药物相比具有卓越的品质,研究了其对脓毒症诱导的ARDS的潜在保护作用。
■将40只Sprague-Dawley大鼠随机分为4组,包括脓毒症+生理盐水组,假手术+生理盐水组,假手术+瑞马唑兰组和脓毒症+瑞马唑兰组。取大鼠肺组织HE染色评估肺损伤,并计算湿重与干重比(W/D)。促炎因子的水平,抗炎因子,外周血中的CD4+和CD8+T细胞,MDA,MPO,用ELISA法测定肺组织中ATP的含量。采用蛋白质印迹法测定肺组织中HMGB1的蛋白表达。
■与假手术+生理盐水和假手术+瑞马佐仑组相比,脓毒症+生理盐水组的W/D比值显著较高,肺损伤评分,IL-1β,IL-6,TNF-α,PCT,CRP,MDP和MPO,同时表现出较低水平的CD4+和CD8+T淋巴细胞,PaO2、PCO2和ATP。脓毒症+生理盐水组大鼠肺泡壁破裂,间质性肺水肿明显。然而,脓毒症+瑞马唑仑组大鼠肺泡结构改善。此外,脓毒症+瑞马唑仑组HMGB1蛋白表达低于脓毒症+生理盐水组.
■雷唑仑可以减轻感染大鼠的炎症反应,从而减轻肺损伤,提高免疫功能,这可能归因于HMGB1蛋白表达的减少。
UNASSIGNED: Sepsis can disrupt immune regulation and lead to acute respiratory distress syndrome (ARDS) frequently. Remazolam, a fast-acting hypnotic drug with superior qualities compared to other drugs, was investigated for its potential protective effects against sepsis-induced ARDS.
UNASSIGNED: Forty Sprague-Dawley rats were randomly divided into four groups, including the sepsis + saline group, sham operation + saline group, sham operation + remazolam group and the sepsis + remazolam group. Lung tissues of rats were extracted for HE staining to assess lung damage, and the wet weight to dry weight (W/D) ratio was calculated. The levels of proinflammatory factors, anti-inflammatory factors, CD4+ and CD8+ T cells in peripheral blood, MDA, MPO, and ATP in the lung tissue were measured by using ELISA. Western blotting was performed to determine the protein expression of
HMGB1 in lung tissues.
UNASSIGNED: In comparison to the sham operation + saline and sham operation + remazolam groups, the sepsis + saline group exhibited significantly higher values for W/D ratio, lung damage score, IL-1β, IL-6, TNF-α, PCT, CRP, MDP and MPO, while exhibiting lower levels of CD4+ and CD8+ T lymphocytes, PaO2, PCO2, and ATP. The rats in the sepsis + saline group displayed ruptured alveolar walls and evident interstitial lung edema. However, the rats in the sepsis + remazolam group showed improved alveolar structure. Furthermore, the
HMGB1 protein expression in the sepsis + remazolam group was lower than the sepsis + saline group.
UNASSIGNED: Remazolam can alleviate the inflammatory response in infected rats, thereby alleviating lung injury and improving immune function, which may be attributed to the reduction in
HMGB1 protein expression.