腰肌面积(LMA)是重要的产肉性状,在决定car体瘦弱中起着关键作用。全基因组关联研究(GWAS)和RNA测序(RNA-seq)分析用于鉴定北京黑猪的候选LMA基因,在中国北方消费者中很受欢迎的品种。susscrofa染色体(SSC)9中的10个单核苷酸多态性(SNPs)与LMA显着相关。这些SNP被映射到2.90Mb(84.94-87.84Mb)区域。共有11个注释基因被定位在该区域,即MEOX2,CRPPA,SOSTDC1,LRRC72,ANKMY2,BZW2,TSPAN13,AGR2,AHR,SNX13和HDAC9。此外,RNA-seq分析在高和低LMA组之间进行,并鉴定了329个差异表达基因(DEGs)。Further,基于DEGs的《京都基因和基因组百科全书》分析显示,JAK/STAT信号通路和催产素信号通路可能与LMA有关。GWAS和RNA-seq分析都确定了HDAC9基因,表明它可能是影响北京黑猪LMA的重要候选基因。这些发现为影响猪LMA含量的机制提供了有价值的分子见解,可用于有针对性的方法,以提高肉类质量和商业盈利能力。
Loin muscle area (LMA) is an important meat production trait and plays a key role in determining carcass leanness. Genome-wide association
study (GWAS) and RNA sequencing (RNA-seq) analysis were used to identify candidate LMA genes in Beijing Black pigs, a popular breed among consumers in northern China. Ten single nucleotide polymorphisms (SNPs) in sus scrofa chromosome (SSC) 9 were significantly associated with LMA. These SNPs were mapped to a 2.90 Mb (84.94-87.84 Mb) region. A total of 11 annotated genes were mapped on this region, namely MEOX2, CRPPA, SOSTDC1, LRRC72, ANKMY2, BZW2, TSPAN13, AGR2, AHR, SNX13, and
HDAC9. In addition, RNA-seq analysis was performed between the high- and low-LMA groups, and 329 differentially expressed genes (DEGs) were identified. Further, Kyoto Encyclopedia of Genes and Genomes analysis based on DEGs revealed that the JAK/STAT signaling pathway and oxytocin signaling pathway may be responsible for LMA. Both GWAS and RNA-seq analysis identified the
HDAC9 gene, indicating that it may be an important candidate gene affecting LMA in Beijing Black pigs. The findings provide valuable molecular insights into the mechanisms that influence LMA content in pigs, which can be utilized in targeted approaches to enhance meat quality and commercial profitability.