糖组学实验所需的最低信息(MIRAGE)是一项标准化糖分析方法报告并评估其可重复性的举措。迄今为止,MIRAGE委员会发布了一些报告指南,描述了样品制备方法应提供的信息,质谱方法,液相色谱分析,外切糖苷酶消化,聚糖微阵列方法,和核磁共振方法。这里,我们提供了第一版的毛细管电泳(CE)分析糖蛋白(proteo)mics报告指南,用于科学文献中实验条件的标准化和高质量报告。该指南涵盖了glyco(proteo)micsCE实验的所有方面,包括样品制备,CE操作模式(CZE,CGE,CEC,MEKC,首席执行官,cITP),仪器配置,毛细管分离条件,检测,数据分析,和实验描述符。这些指南与其他MIRAGE指南链接,可通过项目网站https://www免费获得。贝尔斯坦-研究所。de/en/projects/mirage/guidelines/#ce_analysis(doi:10.3762/mirage.7)。
The Minimum Information Required for a
Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. To date, the MIRAGE Commission has published several reporting
guidelines that describe what information should be provided for sample preparation methods, mass spectrometry methods, liquid chromatography analysis, exoglycosidase digestions, glycan microarray methods, and nuclear magnetic resonance methods. Here, we present the first version of reporting
guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE) for standardized and high-quality reporting of experimental conditions in the scientific literature. The
guidelines cover all aspects of a glyco(proteo)mics CE experiment including sample preparation, CE operation mode (CZE, CGE, CEC, MEKC, cIEF, cITP), instrument configuration, capillary separation conditions, detection, data analysis, and experimental descriptors. These
guidelines are linked to other MIRAGE
guidelines and are freely available through the project website https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis (doi:10.3762/mirage.7).