本研究旨在探讨枸杞和丹参(LFSMR)的作用,一对药物具有滋阴功能,促进血液循环,和明亮的眼睛,通过抑制Müller细胞(MC)的神经胶质增生并诱导其重编程和分化为各种类型的视网膜神经细胞来治疗视网膜色素变性(RP)。12只C57小鼠作为正常对照组,48只转基因RP(rd10)小鼠随机分为模型组,阳性对照组,以及低剂量和高剂量LFSMR组,每组12只小鼠。HE染色检测视网膜病理变化,视网膜电图用于检测视网膜功能。采用视网膜光学相干断层扫描检测视网膜厚度并进行眼底照相,激光散斑灌注成像用于检测局部视网膜血流。数字PCR检测视网膜神经细胞相关基因表达,免疫荧光法检测视网膜神经细胞相关蛋白的表达。LFSMR能显著改善病理改变,增加a波和b波的振幅,增加视网膜厚度,恢复视网膜损伤,并增加RP病变小鼠的视网膜血流量。LFSMR还可以在RP的发病过程中显著抑制胶质纤维酸性蛋白(GFAP)的mRNA表达,并上调性别决定区Y盒蛋白2(SOX2)的mRNA表达,配对盒蛋白6(Pax6),视紫红质,蛋白激酶C-α(PKCα),语法素,和胸腺细胞抗原1.1(Thy1。1).LFSMR能显著抑制GFAP蛋白表达,增强SOX2、Pax6、PKCα,语法素,Thy11.它还可以逆转rd10小鼠视网膜的病理变化,改善视网膜功能和眼底表现,增加视网膜厚度,增强局部视网膜血流,并对RP发挥治疗作用。LFSMR的作用机制可能与抑制MCs的胶质增生、促进MCs重编程和分化为各种类型的视网膜神经细胞有关。
This
study aims to explore the effect of Lycii Fructus and Salviae Miltiorrhizae Radix et Rhizoma(LFSMR), a drug pair possesses the function of nourishing Yin, promoting blood circulation, and brightening the eyes, in treating retinitis pigmentosa(RP)by inhibiting the
gliosis of Müller cells(MCs) and inducing their reprogramming and differentiation into various types of retinal nerve cells. Twelve C57 mice were used as the normal control group, and 48 transgenic RP(rd10) mice were randomly divided into the model group, positive control group, and low and high dose LFSMR groups, with 12 mice in each group. HE staining was used to detect pathological changes in the retina, and an electroretinogram was used to detect retinal function. Retinal optical coherence tomography was used to detect retinal thickness and perform fundus photography, and laser speckle perfusion imaging was used to detect local retinal blood flow. Digital PCR was used to detect gene expression related to retinal nerve cells, and immunofluorescence was used to detect protein expression related to retinal nerve cells. LFSMR could significantly improve the pathological changes, increase the amplitude of a and b waves, increase the retinal thickness, restore retinal damage, and increase retinal blood flow in mice with RP lesions. LFSMR could also significantly inhibit the m RNA expression of the glial fibrillary acidic protein( GFAP) during the pathogenesis of RP and upregulate m RNA expression of sex determining region Y box protein 2(SOX2), paired box protein 6(Pax6),rhodopsin, protein kinase C-α(PKCα), syntaxin, and thymic cell antigen 1. 1(Thy1. 1). LFSMR could significantly inhibit GFAP protein expression and enhance protein expression of SOX2, Pax6, rhodopsin, PKCα, syntaxin, and Thy1. 1. It could also reverse the pathological changes in the retina of rd10 mice, improve retinal function and fundus performance, increase retinal thickness, enhance local retinal blood flow, and exert therapeutic effects on RP. The mechanism of action of LFSMR may be related to inhibiting the
gliosis of MCs and promoting their reprogramming and differentiation into various types of retinal nerve cells.