本研究旨在评估埃及哮喘儿童的血清维生素D和免疫球蛋白E（IgE）水平，并找出维生素D受体（VDR）多态性与支气管哮喘发展的可能关联。
这项研究包括了100名埃及儿童，50名哮喘儿童与50岁相当，性别,和体重指数匹配，使用全球哮喘倡议对哮喘儿童进行非相关健康对照(HCs)临床评估.肺功能检查（1秒用力呼气量[FEV1]，强制肺活量[FVC]，进行FEV1/FVC比率）。进行血清维生素D3和总IgE的酶联免疫吸附测定。VDR-单核苷酸多态性（SNP）（ApaI，TaqI,和BsmI）检测已通过限制性片段长度多态性技术使用聚合酶链反应进行。使用SPSS20.0版进行数据分析。所研究的SNP遵循HardyWeinberg方程。
哮喘儿童的平均血清25（OH）D3水平（13.46ng/mL±10.50SD）明显低于HCs（37.53ng/mL±13。0.40SD)，P<0.05。在72％的病例中检测到维生素D缺乏，其在哮喘控制方面的水平没有显着差异。哮喘儿童的IgE水平（99.83ku/L±233.81SD）明显高于HCs（7.52ku/L±3.32SD），P<0.05。哮喘儿童更常见于TaqIt等位基因(比值比[OR]，2.25；95%置信区间[CI]，1.28-3.96；P<.05）和BsmIb等位基因（OR，1.83；95%CI，1.05-3.21；P<0.05）。ApaIa等位基因在患者与对照组之间没有显着差异（P>0.05）。TT+Tt和Bb+bb基因型在病例中明显高于对照组,所有P<0.05。
TaqI和BsmI与埃及儿童支气管哮喘发展的风险相关。哮喘儿童中经常发生高IgE和低维生素D状态。
The current study aimed to assess the serum levels of vitamin D and immunoglobulin E (IgE) among asthmatic Egyptian children and to find out the possible associations of vitamin D receptor (VDR) polymorphisms with bronchial asthma development.
The study included 100 Egyptian children, 50 asthmatic children who were comparable with 50 age, sex, and body mass index-matched, unrelated healthy controls (HCs) clinical assessments of asthmatic children were done using global initiative of asthma. Pulmonary function tests (forced expiratory volume in 1 second [FEV1], forced vital capacity [FVC], FEV1/FVC ratio) were performed. Enzyme-linked immunosorbent assays of serum vitamin D3 and total IgE were done. VDR-single nucleotide polymorphisms (SNPs) (ApaI, TaqI, and BsmI) detection has performed using polymerase chain reaction through restriction fragment length polymorphism technique. Data analysis was performed using SPSS version 20.0. The studied SNPs were followed the Hardy Weinberg equation.
The mean serum level of 25(OH) D3 was significantly lower among asthmatic children (13.46 ng/mL ± 10.50 SD) in comparison to HCs (37.53 ng/mL ± 13. 0.40 SD), P < .05. Vitamin D deficiency was detected in 72% of cases with no significant difference in its level regarding asthma control. There was significantly higher IgE level among asthmatic children (99.83 ku/L ± 233.81 SD) versus HCs (7.52 ku/L ± 3.32 SD), P < .05. Asthmatic children were presented more commonly with TaqI t allele (odds ratio [OR], 2.25; 95% confidence interval [CI], 1.28-3.96; P < .05) and BsmI b allele (OR, 1.83; 95% CI, 1.05-3.21; P < .05). ApaI a allele was not significantly different among patients versus controls (P > .05). TT + Tt and Bb + bb genotypes were significantly higher among cases versus the controls, P < .05 for all.
TaqI and BsmI were associated with risk of bronchial asthma development among Egyptian children. High IgE and Low vitamin D status were frequently occurring among asthmatic children.
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