目的:基因雄性不育(GMS)系统是植物产生杂种优势的重要策略。为了更好地了解脂质和糖代谢的重要作用,并确定花粉发育和雄性不育的其他候选者,使用甘蓝型油菜中1205AB的GMS系的转录组和代谢组分析作为案例研究。
■为了表征GMS系统,转录组和代谢组谱产生了24个样本和48个样本1205AB。分别。转录组分析产生了总共156.52Gb的干净数据,并揭示了109,541个基因和8,501个新基因的表达水平。此外,在代谢组学分析中总共检测到1,353种代谢物,包括784在正离子模式和569在负离子模式。
结果:从不同比较组中总共鉴定出15,635个差异表达基因(DEGs)和83个差异代谢产物(DMs),其中大部分涉及脂质和糖代谢。结合转录组和代谢组分析发现49个与脂质代谢相关的直系同源GMS基因和46个与糖代谢相关的直系同源GMS基因,以及45个新基因。
■转录组和代谢组谱及其分析为将来发现其他GMS基因和开发更强大的雄性不育育种系统提供了有用的参考数据,用于生产植物杂种。
OBJECTIVE: The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a
case study.
UNASSIGNED: To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
RESULTS: A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
UNASSIGNED: The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids.