查塔姆群岛拥有新西兰一些最珍贵的黑脚鲍鱼(Haliotisiris)床。这种管理良好的渔业包括对渔获量和规模限制的限制,选择性捕鱼方法,和贝类管理。然而,最近生物量和生长参数的下降促使组学研究表征鲍鱼的生物学反应,可能有助于动物管理策略。
这项研究的目的是表征缓慢和快速生长的代谢产物谱,少年鲍鱼和成年鲍鱼,与支持能量代谢的代谢物有关。
气相色谱-质谱代谢物分析,应用氯甲酸甲酯烷基化,对从达勒姆角和瓦雷考里遗址收集的幼年和成年鲍鱼样本进行了研究,查塔姆群岛,新西兰。
从血淋巴和肌肉样本获得的结果表明,来自快速生长地区的鲍鱼,Wharekauri,通过碳水化合物来源促进代谢功能,为脂肪酸和氨基酸合成提供能量。相反,较高的氨基酸水平主要用于促进该人群的增长。幼年鲍鱼的代谢有利于合成代谢,代谢产物从糖酵解和三羧酸循环中转移,用于生产核苷酸,氨基酸和脂肪酸。
这项研究为鲍鱼种群提供了独特的生理见解,支持使用代谢组学作为研究与生长相关的代谢过程的工具。这项工作为未来的工作奠定了基础,旨在开发用于生长和健康监测的生物标志物,以支持不断增长和更可持续的鲍鱼渔业。
BACKGROUND: The Chatham Islands has some of the most prized black-footed abalone (Haliotis iris) beds in New Zealand. This well-managed fishery includes restrictions on catch and size limits, selective fishing methods, and shellfish management. However, recent declines in biomass and growth parameters have prompted omics research to characterise the biological responses of abalone, potentially contributing towards animal management strategies.
OBJECTIVE: The aim of this study was to characterise the metabolite profiles of slow and fast growing, juvenile and adult abalone, relating to metabolites supporting energy metabolism.
METHODS: A gas chromatography-mass spectrometry metabolite profiling, applying methyl chloroformate alkylation, was performed on juvenile and adult abalone samples collected from Point Durham and Wharekauri sites, Chatham Islands, New Zealand.
RESULTS: The results obtained from haemolymph and muscle samples indicated that abalone from the fast-growing area, Wharekauri, fuelled metabolic functions via carbohydrate sources, providing energy for fatty acid and amino acid synthesis. Conversely, higher amino acid levels were largely utilised to promote growth in this population. The metabolism of juvenile abalone favoured anabolism, where metabolites were diverted from glycolysis and the tricarboxylic acid cycle, and used for the production of nucleotides, amino acids and fatty acids.
CONCLUSIONS: This research provides unique physiological insights towards abalone populations supporting the use of metabolomics as a tool to investigate metabolic processes related to growth. This work sets the stage for future work aimed at developing biomarkers for growth and health monitoring to support a growing and more sustainably abalone fishery.