背景:新冠肺炎感染于2020年3月11日被世卫组织宣布为全球大流行。已知GRP78蛋白参与多种病毒的入侵。我们目前的研究试图为新冠肺炎患者GRP78蛋白水平的变化提供一些见解,Covid-19(+)肺炎,与正常人群相比,CT阴性的Covid-19感染病例更多。
方法:42例Covid-19(-)肺炎患者;72例Covid-19感染患者(30例肺炎,42例CT阴性患者)和30例无已知疾病的患者(对照组)在临床和放射学评估后纳入研究。通过市售的酶联免疫吸附测定(ELISA)试剂盒测量受试者的血清GRP78水平。
结果:发现新冠肺炎感染组的GRP78水平显著高于新冠肺炎(-)肺炎组和对照组(分别为p=0.031和p=0.0001)。Covid-19(-)肺炎之间没有显着差异,就GRP78水平而言,Covid-19(+)肺炎和CT阴性Covid19感染组(p=0.09)。此外,Covid-19(-)肺炎组的GRP78水平显著高于对照组(p=0.0001).
结论:这项前瞻性病例对照研究表明,与新冠肺炎和对照组相比,新冠肺炎感染期间血清GRP78水平显著升高。随着SARS-CoV-2病毒与GRP78蛋白之间的联系越来越清楚,这种关联可能会成为治疗目标。
BACKGROUND: Covid-19 infection was declared a global pandemic by WHO on March 11, 2020.
GRP78 protein is known to be involved in the intrusion of numerous viruses. Our current study tries to provide some insight into the variation of
GRP78 protein levels in patients with Covid-19 (-) pneumonia, Covid-19 (+) pneumonia, and CT negative Covid-19 infection in comparison to the normal population through a larger number of cases.
METHODS: 42 patients who have Covid-19 (-) pneumonia; 72 patients who have Covid-19 infection (30 pneumonia,42 CT negative patients) and 30 patient who have no known diseases (control group) have included in the study after the clinical and radiological evaluation. Serum GRP78 levels of the subjects were measured through a commercially available enzyme-linked immunosorbent assay (ELISA) kit.
RESULTS: The
GRP78 level was found to be significantly higher in the Covid-19 infection group than both Covid-19 (-) pneumonia and control group (p = 0.031 and p = 0.0001, respectively).No significant difference was evident between Covid-19 (-) pneumonia, Covid-19 (+) pneumonia and CT negative Covid 19 infection groups with respect to GRP78 levels (p = 0.09). In addition, the
GRP78 levels were significantly higher in the Covid-19 (-) pneumonia group than the control group (p = 0.0001).
CONCLUSIONS: This prospective
case-control study reveals that the serum
GRP78 levels significantly increased during Covid-19 infection in comparison to both the Covid-19 (-) pneumonia and the control group. As the association between SARS-CoV-2 virus and GRP78 protein is revealed more clearly, this association may come to the fore as a therapeutic target.