目的:慢性乙型肝炎(CHB)感染是肝细胞癌(HCC)的主要危险因素。RECK基因是一种关键的抑癌基因。本研究旨在评估RECK基因单核苷酸多态性(SNP)与埃及慢性乙型肝炎患者HCC发展之间的关联。
方法:在本病例对照研究中,我们从消化内科招募CHB患者,Benha大学,从2016年6月到2018年2月。RECK基因SNPrs10814325通过TaqManSNP基因分型测定使用实时PCR等位基因鉴别(应用生物系统,美国)。
结果:我们在这项研究中招募了140名参与者。参与者分为第一组,仅包括50名CHB参与者,第二组,其中包括50名CHB和HCC参与者,第三组,其中包括40名健康参与者。在HCC患者中发现了显着更高的乙型肝炎病毒DNA病毒血症水平。主要的RECK基因型是T/T等位基因,其次是T/C等位基因;然而,研究组间RECK基因SNPs分布无显著差异。RECK基因SNPs在不同Child类HCC患者中没有统计学上的显著差异,site,HCC的大小,淋巴结受累.受试者工作特征曲线显示,血清甲胎蛋白水平为92ng/ml,对HCC的检测敏感性为96%,特异性为100%。工作特性曲线下面积为0.98。
结论:RECK基因SNP与埃及患者乙型肝炎相关HCC的发展和特征没有显着关联。
OBJECTIVE: Chronic hepatitis B (CHB) infection is a major risk factor for hepatocellular carcinoma (HCC). The RECK gene is a critical tumor suppressor gene. This study aimed to assess the association between RECK gene single nucleotide polymorphisms (SNPs) and the development of HCC in Egyptian patients with chronic hepatitis B.
METHODS: In this
case-control study, we enrolled patients with CHB from the Gastroenterology Department, Benha University, from June 2016 to February 2018. The RECK gene SNP rs10814325 was identified using real-time PCR allelic discrimination via TaqMan SNP genotyping assays (Applied Biosystems, USA).
RESULTS: We enrolled 140 participants in this study. The participants were divided into Group I, which comprised 50 participants with CHB only, Group II, which comprised 50 participants with CHB and HCC, and Group III, which comprised 40 healthy participants. A significantly higher hepatitis B virus DNA viremia level was found in patients with HCC. The predominant RECK genotype was the T/T allele, followed by the T/C allele; however, no significant difference in the distribution of RECK gene SNPs was found between the study groups. No statistically significant difference in RECK gene SNPs was reported among patients with HCC of different Child classes or based on the number, site, size of HCC, and lymph node involvement. Receiver operating characteristic curves showed that a serum alpha-fetoprotein level of 92 ng/ml was 96 % sensitive and 100 % specific for the detection of HCC, with an area under the operating characteristic curve of 0.98.
CONCLUSIONS: RECK gene SNPs have no significant association with the development and characteristics of hepatitis B-related HCC in Egyptian patients.