由于泪道腺样囊性癌(LACC)预后差,我们旨在研究神经周浸润(PNI)和随之而来的GDNF/GFRα-1/RET蛋白表达异常对LACC复发的影响。
回顾性分析2001年至2017年间纳入的51例经组织学证实的LACC患者的临床病理资料。应用苏木精和伊红染色来评估PNI。基于组织的免疫组织化学(IHC)检测GDNF,GFRα-1和RET蛋白在福尔马林固定的LACC上进行,石蜡包埋的标本。我们生成了IHC结果的半定量数据,并将其与51例患者的临床病理数据进行了比较。
在51名患者中,19例(37.3%)PNI阳性。有PNI的LACC比没有PNI的LACC更常见(73.7%与37.5%,P=0.01)。GDNF,GFRα-1和RET蛋白表达率为62.7%,62.7%,51例LACC患者中54.9%,分别。所有3种蛋白的表达在有PNI的患者中比没有PNI的患者更常见。与先前的调查结果一致,PNI相关GFRα-1和RET阳性,通过IHC检测,仍然与复发显着相关,而GDNF表达,通过IHC检测,与LACC复发无关。具体来说,GFRα-1和RET同时表达的患者可能具有较高的PNI风险(89.5%的阳性率)和复发风险(84.2%的阳性率).
PNI可能导致LACC复发。GFRα-1和RET蛋白的同时表达,通过IHC检测,可能与LACCPNI和复发有关。
Because of the poor prognosis of lacrimal adenoid cystic carcinoma (LACC), we aimed to investigate the effects of perineural invasion (PNI) and consequent aberrations in
GDNF/GFRα-1/RET protein expression on LACC recurrence.
Clinicopathological data for 51 histologically confirmed patients with LACC enrolled between 2001 and 2017 were retrospectively analyzed. Hematoxylin and eosin staining was applied to assess PNI. Tissue-based immunohistochemistry (IHC) detection of
GDNF, GFRα-1, and RET proteins was performed on LACC formalin-fixed, paraffin-embedded specimens. We generated semi-quantitative data of the IHC results and compared them with the clinicopathological data for the 51 patients.
Of the 51 patients, 19 (37.3%) were PNI positive. Recurrence was more common for LACC with than without PNI (73.7% vs. 37.5%, P = 0.01).
GDNF, GFRα-1, and RET proteins were expressed in 62.7%, 62.7%, and 54.9% of the 51 patients with LACC, respectively. The expression of all 3 proteins was more common in patients with than without PNI. In agreement with previous findings, PNI-associated GFRα-1 and RET positivity, as detected by IHC, remained significantly associated with recurrence, whereas
GDNF expression, as detected by IHC, was not correlated with LACC recurrence. Specifically, patients with concurrent GFRα-1 and RET expression may have a high risk of PNI (89.5% positivity rate) and recurrence (84.2% positivity rate).
PNI may contribute to LACC recurrence. The concurrent expression of GFRα-1 and RET proteins, as detected by IHC, may potentially be associated with LACC PNI and recurrence.