从DDT污染的农业土壤中分离出的木霉FBL587是一种出色的菌株,具有抗DDT和增强土壤中DDT降解过程的能力。这里,在暴露于DDT的情况下,对HamatumFBL587进行了全基因组测序和RNA-Seq研究.在哈玛目FBL587的38.9Mb基因组中,预测并注释了10,944个蛋白质编码基因,包括与真菌修复相关的那些,如次级代谢产物和铁载体的生产。T.hamatumFBL587对DDT暴露的基因组尺度转录反应显示1706个上调的基因,其中一些推测参与了DDT的细胞易位和降解。关于滴滴涕去除能力,发现代谢酶如P450的上调,以及潜在的下游DDT转化酶,如环氧化物水解酶,FAD依赖性单加氧酶,糖基化和谷胱甘肽转移酶。基于转录反应,DDT降解途径可能包括DDT的跨膜转运蛋白,由于DDT暴露引起的氧化应激的抗氧化酶,以及脂肪酶和生物表面活性剂用于增强DDT的溶解度。我们的研究提供了在暴露于DDT的情况下,有关T.hamatumFBL587的第一个基因组和转录组数据,这是更好地了解DDT污染场所的真菌修复策略的基础。
Trichoderma hamatum FBL 587 isolated from DDT-contaminated agricultural soils stands out as a remarkable strain with DDT-resistance and the ability to enhance DDT degradation process in soil. Here, whole genome sequencing and RNA-Seq studies for T. hamatum FBL 587 under exposure to DDT were performed. In the 38.9 Mb-genome of T. hamatum FBL 587, 10,944 protein-coding genes were predicted and annotated, including those of relevance to mycoremediation such as production of secondary metabolites and siderophores. The genome-scale transcriptional responses of T. hamatum FBL 587 to DDT exposure showed 1706 upregulated genes, some of which were putatively involved in the cellular translocation and degradation of DDT. With regards to DDT removal capacity, it was found upregulation of metabolizing enzymes such as P450s, and potentially of downstream DDT-transforming enzymes such as epoxide hydrolases, FAD-dependent monooxygenases, glycosyl- and glutathione-transferases. Based on transcriptional responses, the DDT degradation pathway could include transmembrane transporters of DDT, antioxidant enzymes for oxidative stress due to DDT exposure, as well as lipases and biosurfactants for the enhanced solubility of DDT. Our
study provides the first genomic and transcriptomic data on T. hamatum FBL 587 under exposure to DDT, which are a base for a better understanding of mycoremediation strategies for DDT-polluted sites.