Polygoni Multiflori Radix is a commonly used traditional Chinese medicine in clinical treatment and health care. However, the related liver injury has been frequently reported, which has attracted extensive attention. Fortunately, in recent years, the scholars have made substantial progress in revealing the basic properties, main causes, material basis and molecular mechanism of Polygoni Multiflori Radix related liver injury. The basic characteristics and biomarkers of its susceptible subjects have been found. It indicated that Polygoni Multiflori Radix is only at risk of liver injury for a small number of specific subjects but safe for the majority. To provide detailed guidance for the rational use of Polygoni Multiflori Radix and related preparation, we compiled this guideline based on existing research and knowledge. The guideline has been issued by the China Association of Chinese Medicine(No. T/CACM 1328-2019).