由CRISPR/Cas9系统产生的MSTN和FGF5基因敲除绵羊表现出“双肌”表型，增加了头发的密度和长度，提供有价值的新育种材料。在之前的研究中,我们获得了具有显著育种价值的MSTN和FGF5双敲除绵羊。在这项研究中,我们在Wistar大鼠中进行了90天喂养研究,以评估基因组编辑羊肉的安全性.7只大鼠组，每组10只雌性和10只雄性，分别喂食不同浓度（3.75％，7.5%,和15％）的双敲除羊肉或野生型羊肉在常规商业饮食中持续90天。在喂食结束时，进行尿常规、血常规检查及血液生化指标测定。此外，对各组大鼠的主要器官进行称重和组织病理学检查。尽管两组在某些参数上存在显着差异，所有值均在正常范围内.因此,90天的大鼠饲喂研究表明，来自MSTN和FGF5双敲除绵羊的肉对大鼠健康没有任何长期不利影响。本研究也为多基因敲除动物肉的安全性评估提供了有价值的参考信息。
MSTN and
FGF5 gene knockout sheep generated by the CRISPR/Cas9 system exhibit the \'double-muscle\' phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous
study, we obtained MSTN and
FGF5 double-knockout sheep of significant breeding value. In this
study, we carried out a 90-day feeding
study in Wistar rats to assess the safety of genome-edited mutton. Seven rat groups with 10 females and 10 males per group were fed different concentrations (3.75%, 7.5%, and 15%) of double-knockout mutton or wild-type mutton in a conventional commercial diet for 90 days. At the end of the feeding, routine urine and blood tests and measurements of blood biochemical indicators were performed. Furthermore, the major organs of each group of rats were weighed and examined histopathologically. Although there were significant differences among the groups in some parameters, all values were within the normal ranges. Therefore, the 90-day rat feeding
study showed that the meat from MSTN and
FGF5 double-knockout sheep did not have any long-term adverse effects on rat health. This
study also provides valuable reference information for assessing the safety of meat from animals with knockout of multiple genes.
