背景:药用植物是植物化学物质的来源,它们因其有效性而用于治疗几种氧化应激相关疾病或其他疾病。低毒性和容易获得。西孟加拉邦五种传统上使用且特征较少的草本杂草,印度,即,菊胺,TridaxProcumbens,清晰的红宝石,康美利纳和大猩猩,为当前的研究进行了调查。方法:分析了叶片的水提取物和70%乙醇提取物,以估算必需的植物化学物质并评估其体外抗氧化状态。药用特性和细胞毒性作用。据我们所知,首次报道了使用这些草药的几种分析和比较评估。对于定量研究,采用紫外-可见分光光度法和带二极管阵列检测器的高效液相色谱HPLC-DAD技术。使用Kirby-Bauer圆盘扩散方法研究了抗菌性能。对于体外抗结石研究,使用了滴定法。使用外周血单核细胞进行细胞活力测定。结果:水提物中的多酚含量较高,黄酮类化合物,单宁和自由基清除试验的抑制百分比值,而70%的乙醇提取物表现出更高的生物碱和强心苷含量。对70%乙醇提取物的HPLC-DAD分析使我们鉴定了10种主要的酚类成分。一品红提取物显示出最小的细胞毒性(细胞死亡〜2.5%和4%的水和70%的乙醇提取物,分别),而Cleomerutidosperma和Tridaxprocumbens\'70%乙醇提取物显示更高的细胞死亡(~13%和28%,分别),与对照组相比(细胞死亡〜10-12%)。结论:该研究得出结论,在本研究中选择的所有药用杂草中,一品红具有最高量的生物活性化合物,因此表现出最高的体外抗氧化活性和有希望的体外药用特性。
Background: Medicinal plants are a source of phytochemicals and they are used for the treatment of several oxidative stress-related or other diseases for their effectiveness, low toxicity and easy availability. Five traditionally used and less characterized herbaceous weeds of West Bengal, India, namely, Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commelina benghalensis and
Euphorbia hirta, were investigated for the current research
study. Methods: Aqueous and 70% ethanolic extracts of the leaves were analyzed for estimation of essential phytochemicals and to evaluate their in vitro antioxidant status, medicinal properties and cytotoxic effects. To the best of our knowledge, several assays and comparative evaluations using these herbs are reported for the first time. For quantitative study, UV-vis spectrophotometry and high-performance liquid chromatography with diode array detector HPLC-DAD techniques were used. Antibacterial properties were investigated using the Kirby-Bauer disc diffusion method. For in vitro anti-lithiatic
study, a titration method was used. The cell viability assay was done using peripheral blood mononuclear cells. Results: The aqueous extract exhibits higher content of polyphenols, flavonoids, tannins and inhibition percentage values for free radical scavenging assays, whereas the 70% ethanolic extract exhibits higher content of alkaloids and cardiac glycosides. HPLC-DAD analysis of 70% ethanolic extracts led us to identify 10 predominant phenolic constituents.
Euphorbia hirta extracts showed minimum cytotoxicity (cell death ~2.5% and 4% in water and 70% ethanolic extract, respectively ), whereas Cleome rutidosperma and Tridax procumbens\' 70% ethanolic extracts showed higher cell death (~13% and 28%, respectively), compared with the control (cell death ~10-12%). Conclusions: The
study concluded that of all the medicinal weeds selected for the current
study,
Euphorbia hirta possesses the highest amount of bioactive compounds and hence exhibits the highest in vitro antioxidant activity and promising in vitro medicinal properties.