■探索表观遗传规则,比如microRNA,在新生儿中,对于增强我们解决和预防早期发育迟缓的能力具有重要的希望。
■因此,这项研究旨在调查miRNA在婴儿脐带血中的表达是否可以预测其随着年龄增长的发育结果。
■我们注册了143名足月新生儿,通过剖宫产(CS)或自然自然分娩(NSD)分娩。然后我们分析了特定miRNA的概况(miR-486-5p,miR-126-5p,miR-140-3p,miR-151a-3p,miR-142-5p,和miR-30e-5p)在这些婴儿的脐带血中。随后,当队列达到1岁时,我们使用Bayley-III评分进行随访评估.此外,我们对与这些检测的miRNA相关的靶基因进行了通路富集分析.
■将通过剖宫产(CS)分娩的新生儿与通过自然自然分娩(NSD)出生的新生儿进行比较时,我们观察到显著的差异。具体来说,通过NSD的新生儿对miR-486-5p显示出明显更高的ΔCt值,此外,脐带血中miR-126-5p和miR-151a-3p的ΔCt值较低。一岁时,认知发育与miR-140-3p和miR-142-5p的ΔCt值显着相关,而语言发育显示与miR-140-3p的ΔCt值显著相关。此外,我们的通路富集分析显示,这些miRNAs的靶基因始终参与与神经元相关的通路,如轴突导向和神经营养蛋白信号通路。
■总之,这项研究在阐明脐带血miRNA水平与1岁新生儿健康指标和神经发育之间的潜在联系方面是一项开创性工作.我们的发现强调了出生时miRNA水平在影响神经发育相关机制中的重要性。
UNASSIGNED: Exploring the epigenetic regulations, such as microRNA, in newborns holds significant promise for enhancing our ability to address and potentially prevent early-life developmental delays.
UNASSIGNED: Hence, this research seeks to investigate if the expression of miRNA in the umbilical cord blood of infants can forecast their developmental outcomes as they grow older.
UNASSIGNED: We enrolled 143 full-term newborns, delivered either via cesarean section (CS) or through natural spontaneous delivery (NSD). We then analyzed the profiles of specific miRNAs (miR-486-5p, miR-126-5p, miR-140-3p, miR-151a-3p, miR-142-5p, and miR-30e-5p) in the umbilical cord blood of these infants. Subsequently, we performed follow-up assessments using Bayley-III scores when the cohort reached 1 year of age. Furthermore, we conducted pathway-enrichment analyses on the target genes associated with these examined miRNAs.
UNASSIGNED: When comparing newborns delivered via cesarean section (CS) to those born via natural spontaneous delivery (NSD), we observed notable differences. Specifically, newborns through NSD displayed significantly higher ΔCt values for miR-486-5p, alongside lower ΔCt values for miR-126-5p and miR-151a-3p in their cord blood. At 1 year of age, cognitive development was significantly linked to the ΔCt values of miR-140-3p and miR-142-5p, while language development showed a significant association with the ΔCt values of miR-140-3p. Moreover, our pathway enrichment analyses revealed that the target genes of these miRNAs were consistently involved in the pathways related to neurons, such as axon guidance and the neurotrophin signaling pathway.
UNASSIGNED: In summary, this
study represents a pioneering effort in elucidating the potential connections between miRNA levels in cord blood and the health indicators and neurodevelopment of newborns at 1 year of age. Our findings underscore the significance of miRNA levels at birth in influencing mechanisms related to neurodevelopment.