本文旨在研究全血淋巴细胞中EB病毒核酸定量（EBV-DNA）的结果，以检查血清是否可以诊断儿童的EB病毒感染。A组包括2017年2月至2018年2月期间住院的65名可能患有EB病毒感染的儿童。在同一时期的健康检查中，随机选择65名儿童作为B组。
为了找出A组和B组之间的差异，采用荧光定量法检测全血淋巴细胞和血清中EBV-DNA,酶联免疫吸附试验检测EBV-CA-IgM抗体。
结果表明，A组的全血淋巴细胞和血清中EBV-DNA阳性率高于B组。A组和B组的全血淋巴细胞中EBV-DNA阳性率高于血清。在A组的全血淋巴细胞中，EBV-DNA和EBV-CA-IgM抗体阳性率有统计学意义。
EBV感染儿童全血淋巴细胞中EBV-DNA阳性率高于血清,应根据儿童患者的个人情况选择合适的诊断标本。
The article aims to
study the results of EB virus nucleic acid quantification (EBV-DNA) in whole blood lymphocytes to examine whether serum can diagnose EB virus infection in children. Group A consisted of 65 children admitted to the hospital with a probable EB virus infection between February 2017 and February 2018. In the same period of health check-ups, 65 children were randomly selected as Group B.
To find out the differences between Group A and Group B, EBV-DNAs in whole blood lymphocyte and serum were detected by quantitative fluorescence assay, and EBV-CA-IgM antibody was detected by enzyme-linked immunosorbent assay.
The results demonstrate that Group A had a greater rate of positive EBV-DNAs in whole blood lymphocytes and serum than Group B. EBV-DNA positivity was greater in whole blood lymphocytes of Group A and Group B than in serum. In whole blood lymphocytes from Group A, the positive rate of EBV-DNA and EBV-CA-IgM antibodies was statistically significant.
The positive rate of EBV-DNA in whole blood lymphocytes of EBV-infected children is higher than in serum, the suitable specimens for diagnosis should be chosen based on the individual condition of children patients.
