■偏心运动可能会触发机械应力,导致肌肉损伤,可能降低运动表现。L-瓜氨酸可预防急性偏心运动后骨骼肌损伤。本研究旨在评估L-瓜氨酸作为急性偏心运动后小鼠骨骼肌损伤的预防性治疗的剂量-反应效应。
■这是一项受控的实验室体内研究,仅采用测试后设计。雄性小鼠(BALB/c,n=25)被随机分为以下组:正常对照(C1)(n=5);阴性对照(C2),下坡跑步和安慰剂干预(n=5);治疗组:T1(n=5),T2(n=5),和T3(n=5),进行下坡跑步和250、500和1,000mg/kg的L-瓜氨酸,分别,七天。采用ELISA法检测血浆中TNNI2和腓肠肌组织NOX2、IL-6和胱天蛋白酶3的水平。免疫组化法检测NF-κB和HSP-70的表达。
■偏心运动后的小鼠骨骼肌损伤(血浆TNNI2水平)在250和500mg/kg的L-瓜氨酸后较低。Further,氧化应激标志物的变化,NOX2在1,000mg/kg剂量后降低。然而,在细胞反应标志物水平上观察到较低水平的变化(NF-κB,HSP-70,IL-6和半胱天冬酶3)在施用250、500和1,000mg/kg的L-瓜氨酸剂量后。
■L-瓜氨酸可通过抗氧化作用以及炎症和凋亡途径预防急性偏心运动后小鼠骨骼肌损伤。关于剂量相关效应,发现250、500和1,000mg/kg的L-瓜氨酸剂量显着影响NF-κB和HSP-70的表达以及IL-6和caspase3的水平。同时,只有250和500毫克/千克的剂量对TNNI2水平有影响,和1,000mg/kg剂量影响NOX2水平。
UNASSIGNED: Eccentric exercise may trigger mechanical stress, resulting in muscle damage that may decrease athletic performance. L-citrulline potentially prevents skeletal muscle damage after acute eccentric exercise. This
study aimed to assess the dose-response effect of L-citrulline as a preventive therapy for skeletal muscle damage in mice after acute eccentric exercise.
UNASSIGNED: This is a controlled laboratory in vivo
study with a post-test-only design. Male mice (BALB/c, n = 25) were randomized into the following groups: a normal control (C1) (n = 5); a negative control (C2) with downhill running and placebo intervention (n = 5); treatment groups: T1 (n = 5), T2 (n = 5), and T3 (n = 5), were subjected to downhill running and 250, 500, and 1,000 mg/kg of L-citrulline, respectively, for seven days. Blood plasma was used to determine the levels of TNNI2 and gastrocnemius muscle tissue NOX2, IL-6, and caspase 3 using ELISA. NF-κB and HSP-70 expressions were determined by immunohistochemistry.
UNASSIGNED: Skeletal muscle damage (plasma TNNI2 levels) in mice after eccentric exercise was lower after 250 and 500 mg/kg of L-citrulline. Further, changes in oxidative stress markers, NOX2, were reduced after a 1,000 mg/kg dose. However, a lower level of change has been observed in levels of cellular response markers (NF-κB, HSP-70, IL-6, and caspase 3) after administration of L-citrulline doses of 250, 500, and 1,000 mg/kg.
UNASSIGNED: L-citrulline may prevent skeletal muscle damage in mice after acute eccentric exercise through antioxidant effects as well as inflammatory and apoptotic pathways. In relation to dose-related effects, it was found that L-citrulline doses of 250, 500, and 1,000 mg/kg significantly influenced the expression of NF-κB and HSP-70, as well as the levels of IL-6 and caspase 3. Meanwhile, only doses of 250 and 500 mg/kg had an impact on TNNI2 levels, and the 1,000 mg/kg dose affected NOX2 levels.