阿魏酸酯酶(FAE)是能够释放羟基肉桂酸或合成其酯衍生物的多功能酶,这两种分子都具有有趣的生物活性,如:抗氧化剂,抗真菌药,抗病毒药物,抗纤维化,抗炎,在其他人中。这些分子在医学中的重要性,食品或化妆品行业为FAE提供了几种生物技术应用,作为关键的工业生物催化剂。然而,FAE有一些必须克服的操作限制,可以通过不同的蛋白质工程方法来提高它们的热稳定性,催化效率,和选择性。这篇综述旨在通过用于改善酶性能的诱变策略进行简短的历史考察,并分析当前应用于FAE作为有趣的生物催化剂的蛋白质工程策略。最后,给出了实现成功的工业生物催化剂的FAE蛋白质工程方法的前景。
Feruloyl esterases (FAEs) are versatile enzymes able to release hydroxycinnamic acids or synthesize their ester derivatives, both molecules with interesting biological activities such as: antioxidants, antifungals, antivirals, antifibrotic, anti-inflammatory, among others. The importance of these molecules in medicine, food or cosmetic industries provides FAEs with several biotechnological applications as key industrial biocatalysts. However, FAEs have some operational limitations that must be overcome, which can be addressed through different protein engineering approaches to enhance their thermal stability, catalytic efficiencies, and selectivity. This
review aims to present a brief historical tour through the mutagenesis strategies employed to improve enzymes performance and analyze the current protein engineering strategies applied to FAEs as interesting biocatalysts. Finally, an outlook of the future of FAEs protein engineering approaches to achieve successful industrial biocatalysts is given.