在兽医实践中,许多引起膀胱功能障碍的泌尿系统疾病需要扩大膀胱成形术(AC)。这项研究的目的是评估狗阴道膜同种异体移植物(DTVA),在狗的45±5%膀胱切除术模型后,绵羊阴道膜异种移植物(STVX)和绵羊阴道膜脱细胞细胞外基质(STVDEM)作为膀胱(UB)重建的移植材料。在这项研究中,将18只成年男女健康的杂种犬分为三组(每组6只):DTVA组,STVX组,和STVDEM组。使用临床对不同组的AC进行评估,血液学,血清生化,尿液,超声检查,逆行阳性膀胱造影,和组织病理学分析在整个12周的研究期间。所有组的狗都在手术中幸存下来,除了三只狗死于STVX和DTVA组。平均膀胱容量表明DTVA和STVX组恢复了82.22%和68.62%,分别,他们的术前基线能力。有趣的是,STVDEM组的膀胱容量增加到113.70%。尽管组织学分析显示三个移植物成功重建了膀胱壁,STVDEM表现出组织良好且分化良好的上皮和肌肉组织,但并不完全相同,天然UB组织。因此,STVDEM被认为是狗UB重建的理想和潜在的脱细胞移植物,而DTVA和STVX可用于需要UB重建的紧急情况。
In veterinary practice, numerous urological disorders that cause bladder dysfunction necessitate augmentation cystoplasty (AC). The purpose of this
study is to evaluate the dog tunica vaginalis allograft (DTVA), sheep tunica vaginalis xenograft (STVX) and sheep tunica vaginalis decellularized extracellular matrix (STVDEM) as graft materials for urinary bladder (UB) reconstruction following a 45±5% cystectomy model in dogs. In this
study, 18 adult apparently healthy mongrel dogs of both sexes were divided into three groups (6 dogs each): the DTVA group, the STVX group, and the STVDEM group. The evaluation of the AC in different groups was carried out using clinical, hematological, serum biochemical, urine, ultrasonographic, retrograde positive cystogram, and histopathological analysis all over the
study period of 12 weeks. The dogs in all groups survived the procedures, except three dogs died from both STVX and DTVA groups. The mean bladder capacity indicated that the DTVA and STVX groups had regained 82.22% and 68.62%, respectively, of their preoperative baseline capacity. Interestingly, the STVDEM group\'s bladder capacity increased to 113.70%. Although histological analysis revealed that the three grafts successfully rebuilt the bladder wall, the STVDEM demonstrated well-organized and well-differentiated epithelial and muscular tissues that resembled, but were not identical to, native UB tissues. As a result, STVDEM is proposed as an ideal and potential acellular graft for UB reconstruction in dogs, whereas DTVA and STVX could be employed in emergencies requiring UB reconstruction.