表观遗传变化可能是对环境压力的反应,改变的表观基因组模式可能代表环境暴露的稳定特征。
这里,在一个大型跨国队列中,我们研究了910份诊断前外周血样本中DNA甲基化变化作为烟草烟雾暴露标志物的可能性.
我们确定了748个CpG位点在吸烟者和非吸烟者之间差异甲基化,其中我们确定了与主动吸烟相关的新的区域聚集的CpG。重要的是,我们发现了戒烟后甲基化变化的显著可逆性,尽管特定基因在戒烟后22年仍有差异甲基化。
我们的研究对吸烟相关的DNA甲基化改变进行了全面分类,并表明这些改变在戒烟后是可逆的。
Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure.
Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort.
We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation.
Our
study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.