背景：我们最近基于一组13个基因的DNA甲基化分析，开发了一种用于口腔鳞状细胞癌（OSCC）检测的非侵入性采样程序。口腔癌,以及急性和慢性炎症性疾病，可能影响口腔中几种基因的甲基化水平。在本研究中,我们使用13个基因组评估了牙周病(PD)的存在和甲基化状态.
方法：从三个不同的患者组中收集口腔刷牙标本：23例牙龈OSCC患者，15例受PD影响的患者，和15名缺乏PD证据的健康志愿者。进行DNA甲基化分析,并基于预定义的截止值确定每个样品为阳性或阴性。
结果：在23/23OSCC患者中发现阳性结果，3/15的PD患者，和健康志愿者的0/15样本。PD组中的GP1BB和MIR193基因表现出与OSCC患者相似的平均甲基化水平。ZAP70在三组间显示不同的甲基化水平。
结论：初步数据确定了PD和OSCC患者在两个炎症基因（GP1BB和MIR193）中的共有表观遗传学改变。这项研究可能有助于确定这两种疾病之间的潜在联系，并作为未来研究集中在发病机理上的起点。
BACKGROUND: We recently developed a non-invasive sampling procedure for oral squamous cell carcinoma (OSCC) detection based on DNA methylation analysis of a panel of 13 genes. Oral cancer, as well as acute and chronic inflammatory diseases, may influence the methylation level of several genes in the oral cavity. In the present
study, we evaluated the presence of periodontal disease (PD) and the methylation status using our 13-gene panel.
METHODS: Oral brushing specimens were collected from three different patient groups: 23 gingival OSCC patients, 15 patients affected by PD, and 15 healthy volunteers lacking evidence of PD. DNA methylation analysis was performed and each sample was determined to be positive or negative based on a predefined cut-off value.
RESULTS: Positive results were found for 23/23 OSCC patients, 3/15 PD patients, and 0/15 samples from healthy volunteers. The GP1BB and MIR193 genes in the PD group exhibited mean methylation levels similar to OSCC patients. ZAP70 showed different methylation levels among three groups.
CONCLUSIONS: Preliminary data identified shared epigenetic alterations between PD and OSCC patients in two inflammatory genes (GP1BB and MIR193). This
study may help to identify potential links between the two diseases and serve as a starting point for the future research focused on pathogenesis.
