由于DNA降解,受损骨骼遗骸的法医DNA分析可能会带来挑战,通常导致部分或阴性常染色体STRs谱。为了解决这个问题,可以采用其他方法,如线粒体DNA或SNP分型;然而,它们是劳动密集型和昂贵的。插入无效等位基因(INNULs),短的散布核元素,由于它们的小扩增子大小,已经被认为是在具有挑战性的样品中用于人类鉴定的有价值的工具。已经开发了包括20种INNULs标记以及牙釉质蛋白(InnoTyper®21)的商业试剂盒。这项研究使用降解的骨骼遗骸评估其效用,比较获得的结果(检测到的等位基因数量,RFU值,PHR,和可报告标记的数量)到使用GlobalFiler™获得的那些。随后,使用Familias版本3确定每个样本的两个配置文件的随机匹配概率,以评估从每个试剂盒获得的结果的辨别能力.在每个样本中,InnoTyper®21产生了更多的等位基因,更高的RFU值,和更多的可报告基因座。然而,在大多数情况下,这两个配置文件是类似的信息。总之,InnoTyper®21在获得不良或负面概况的情况下,可作为分析具有挑战性的样品的有价值的补充。
Forensic DNA analysis in compromised skeletal remains may pose challenges due to DNA degradation, often resulting in partial or negative autosomal STRs profiles. To address this issue, alternative approaches such as mitochondrial DNA or SNPs typing may be employed; however, they are labour-intensive and costly. Insertion-null alleles (INNULs), short interspersed nuclear elements, have been suggested as a valuable tool for human identification in challenging samples due to their small amplicon size. A commercial kit including 20 INNULs markers along with amelogenin (InnoTyper® 21) has been developed. This study assesses its utility using degraded skeletal remains, comparing the results obtained (the number of detected alleles, RFU values, PHR, and the number of reportable markers) to those obtained using GlobalFiler™. Subsequently, the random match probability of the two profiles for each sample was determined using Familias version 3 to evaluate the power of discrimination of the results obtained from each kit. In every sample, InnoTyper® 21 yielded more alleles, higher RFU values, and a greater number of reportable loci. However, in most cases, both profiles were similarly informative. In conclusion, InnoTyper® 21 serves as a valuable complement to the analysis of challenging samples in cases where a poor or negative profile was obtained.