受损的自然杀伤(NK)细胞毒性功能是肌痛性脑脊髓炎/慢性疲劳综合征(ME/CFS)的有据可查且一致的特征。在ME/CFS患者的NK细胞中评估的其他结果,然而,保持模棱两可。本研究的目的是对有关NK细胞表型的文献进行系统回顾。受体表达,ME/CFS患者的细胞因子产生和细胞毒性,并确定作为ME/CFS模型的适当性。
Medline(EBSCOHost),Scopus,系统搜索EMBASE和PubMed数据库,以获取1994年至2018年3月发表的相关论文。该综述包括研究ME/CFS患者的NK细胞特征,并在给予特定的纳入和排除标准后与HC进行比较。次要结果包括分离NK细胞的遗传分析或生活质量评估。除了JoannaBriggs研究所清单外,还使用Downs和Black清单完成了质量评估。
本次审查包括17篇合格出版物。所有研究均为观察性病例对照研究。其中,11调查了NK细胞的细胞毒性,14调查了NK细胞表型和受体谱,三个检查NK细胞细胞因子的产生,六个调查NK细胞裂解蛋白水平和四个调查NK细胞脱粒。受损的NK细胞的细胞毒性仍然是所有出版物中最一致的免疫学报告。研究之间的其他结果有所不同。
在这篇综述中包含的所有论文中,一致的发现是NK细胞的细胞毒性受损,提示它是ME/CFS患者持续研究的可靠和合适的细胞模型。还报道了NK细胞裂解蛋白水平的畸变。尽管建议进行额外的研究,当前的研究为后续调查提供了基础。由于疾病本身的异质性以及研究NK功能特定特征的研究之间的发现,NK细胞异常可能用于表征ME/CFS的子集。
Compromised natural killer (NK) cell cytotoxic function is a well-documented and consistent feature of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Other outcomes evaluated in NK cells of ME/CFS patients, however, remain equivocal. The aim of this study was to conduct a systematic
review of the literature regarding NK cell phenotype, receptor expression, cytokine production and cytotoxicity in ME/CFS patients and determine the appropriateness as a model for ME/CFS.
Medline (EBSCOHost), Scopus, EMBASE and PubMed databases were systematically searched to source relevant papers published between 1994 and March 2018. This
review included studies examining NK cells\' features in ME/CFS patients compared with HC following administration of specific inclusion and exclusion criteria. Secondary outcomes included genetic analysis in isolated NK cells or quality of life assessment. Quality assessment was completed using the Downs and Black checklist in addition to The Joanna Briggs Institute checklist.
Seventeen eligible publications were included in this
review. All studies were observational case control studies. Of these, 11 investigated NK cell cytotoxicity, 14 investigated NK cell phenotype and receptor profiles, three examined NK cell cytokine production, six investigated NK cell lytic protein levels and four investigated NK cell degranulation. Impaired NK cell cytotoxicity remained the most consistent immunological report across all publications. Other outcomes investigated differed between studies.
A consistent finding among all papers included in this
review was impaired NK cell cytotoxicity, suggesting that it is a reliable and appropriate cellular model for continued research in ME/CFS patients. Aberrations in NK cell lytic protein levels were also reported. Although additional research is recommended, current research provides a foundation for subsequent investigations. It is possible that NK cell abnormalities can be used to characterise a subset of ME/CFS due to the heterogeneity of both the illness itself and findings between studies investigating specific features of NK function.