■呼吸道有多种微生物群,其组成和丰度取决于特定的场地因素,与外部因素的相互作用,和疾病。这项研究的目的是调查COVID-19严重程度与鼻咽微生物组之间的关系。
■我们在墨西哥城进行了一项前瞻性队列研究,收集30例COVID-19患者和14例健康志愿者的鼻咽拭子。使用16SrRNA基因分析进行微生物组谱分析。分类分配,分类,多样性分析,核心微生物组分析,并使用R包进行统计分析。
微生物组数据分析显示,严重COVID-19的鼻咽微生物组有分类学上的变化。特别是,我们观察到重症COVID-19患者中劳森菌属和镰刀杆菌属的相对丰度显着降低(p<0.001)。相比之下,这些患者表现出明显的链球菌富集,放线菌,肽链球菌,Atobobium,肉芽肿,小杆菌属,Veillonella,Prevotella_7,Rothia,Gemella,Alloprevotella,和单菌属(p<0.01)。对所有样品的核心微生物组的分析一致确定了葡萄球菌的存在,棒状杆菌,和链球菌。
■我们的研究表明,危重病COVID-19患者的炎症过程和插管程序导致的物理化学条件和屏障的破坏可能会促进口腔微生物对鼻咽的定植和入侵。
UNASSIGNED: The respiratory tract harbors a variety of microbiota, whose composition and abundance depend on specific site factors, interaction with external factors, and disease. The aim of this study was to investigate the relationship between COVID-19 severity and the nasopharyngeal microbiome.
UNASSIGNED: We conducted a prospective cohort study in Mexico City, collecting nasopharyngeal swabs from 30 COVID-19 patients and 14 healthy volunteers. Microbiome profiling was performed using 16S rRNA gene analysis. Taxonomic assignment, classification, diversity analysis, core microbiome analysis, and statistical analysis were conducted using R packages.
UNASSIGNED: The microbiome data analysis revealed taxonomic shifts within the nasopharyngeal microbiome in severe COVID-19. Particularly, we observed a significant reduction in the relative abundance of Lawsonella and Cutibacterium genera in critically ill COVID-19 patients (p < 0.001). In contrast, these patients exhibited a marked enrichment of Streptococcus, Actinomyces, Peptostreptococcus, Atopobium, Granulicatella, Mogibacterium, Veillonella, Prevotella_7, Rothia, Gemella, Alloprevotella, and Solobacterium genera (p < 0.01). Analysis of the core microbiome across all samples consistently identified the presence of Staphylococcus,
Corynebacterium, and Streptococcus.
UNASSIGNED: Our study suggests that the disruption of physicochemical conditions and barriers resulting from inflammatory processes and the intubation procedure in critically ill COVID-19 patients may facilitate the colonization and invasion of the nasopharynx by oral microorganisms.